Feed additive for fishes and preparation method thereof
A feed additive and additive technology, applied in the direction of animal feed, animal feed, application, etc., can solve the problem of large effect, achieve the effects of improving utilization rate, preventing mildew and deterioration, and enhancing fish immunity
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Embodiment 1
[0039] Example 1: Fish feed additive and its preparation method
[0040] 1. Composition: Acanthopanax senticosus 15g, Poria mycelium polysaccharide 25.5g, Chinese sea buckthorn 105g, sodium acetate 300g, acetic acid 430g, bamboo vinegar 15g, potassium sorbate 96g, bile acid 2.5g, vitamin C2g, sodium lysine 1g, 8g secondary powder.
[0041] 2. Preparation method:
[0042] 1) Poria Mycelium Polysaccharide is prepared by the following method:
[0043] Poria were purchased from Hunan Institute of Microbiology.
[0044] Configuration standard medium: glucose 4%, peptone 0.75%, potassium nitrate 0.75%, MgSO 4 ·7H 2 O 0.05%, KH 2 PO 4 0.1%, adjusted to pH 5.2, filled liquid volume was 80 mL, culture fermentation temperature was 25°C, shake flask rotation speed was 150 rpm, seed liquid inoculation volume was 5%, fermentation was stopped after 4 days of liquid fermentation in a fermenter. The response surface method was used to extract the polysaccharide from Poria cocos mycelium: the extracti...
Embodiment 2
[0048] Example 2: Fish feed additive and its preparation method
[0049] 1. Composition: Acanthopanax senticosus 13g, Poria mycelium polysaccharide 20g, Chinese sea buckthorn 112.5g, sodium acetate 275g, acetic acid 460g, bamboo vinegar 13g, potassium sorbate 98g, bile acid 2g, vitamin C1.5g, lysine 0.6g sodium, 9.4g secondary powder.
[0050] 2. Preparation method:
[0051] 1) Poria Mycelium Polysaccharide is prepared by the following method:
[0052] Poria were purchased from Hunan Institute of Microbiology.
[0053] Configuration standard medium: glucose 4%, peptone 0.75%, potassium nitrate 0.75%, MgSO 4 ·7H 2 O 0.05%, KH 2 PO 4 0.1%, adjusted to pH 5.5, filled liquid volume was 90 mL, culture fermentation temperature was 26°C, shake flask rotation speed was 150 rpm, seed liquid inoculation volume was 5.5%, fermentation was stopped after 4.5 days of liquid fermentation in a fermentor. The response surface method was used to extract polysaccharides from Poria cocos mycelium: the ext...
Embodiment 3
[0057] Example 3: Fish feed additive and its preparation method
[0058] 1. Composition: Acanthopanax senticosus 20g, Poria mycelium polysaccharide 30g, Chinese sea buckthorn 95.5g, sodium acetate 330g, acetic acid 405g, bamboo vinegar 9g, potassium sorbate 102g, bile acid 3g, vitamin C 2.5g, lysine 1.4g sodium, 6.6g secondary powder.
[0059] 2. Preparation method:
[0060] 1) Poria Mycelium Polysaccharide is prepared by the following method:
[0061] Poria were purchased from Hunan Institute of Microbiology.
[0062] Configuration standard medium: glucose 4%, peptone 0.75%, potassium nitrate 0.75%, MgSO 4 ·7H 2 O 0.05%, KH 2 PO 4 0.1%, adjusted to pH 6.0, filled liquid volume was 240 mL, culture fermentation temperature was 27°C, shake flask rotation speed was 150 rpm, seed liquid inoculation volume was 7%, fermentation was stopped after 6 days of liquid fermentation in a fermentor. The response surface method was used to extract the polysaccharide from Poria cocos mycelium: the ext...
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