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Flow-through fluorescence detection cell

A fluorescence detection and detection cell technology, applied in the field of fluorescence detectors, can solve problems such as high background noise of detection cells, and achieve the effects of reducing high background noise, easy disassembly and replacement of parts, and wide inner diameter range

Active Publication Date: 2015-07-01
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to improve the deficiencies of the prior art, the present invention provides a novel flow-through fluorescence detection cell, which effectively solves the problem of high background noise in the existing detection cell

Method used

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Experimental program
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Effect test

Embodiment 1

[0034] A flow-through fluorescent detection cell, the cross-sectional view is as follows figure 1As shown, it consists of an upper clamp 1, a lower clamp 2 and a cylindrical quartz tube 3. The upper clamp 1 and the lower clamp 2 are oxidized and blackened aluminum materials. The upper clamp 1 is a cylindrical structure, and the lower end surface is provided with a through rectangular upper groove 4; at the bottom of the rectangular upper groove 4, the upper end surface of the clamp 1 is vertically upwardly provided with a through hole as a light hole 5, The depth of the light hole 5 reaches the bottom of the upper groove 4; the lower clamp 3 is a cylindrical structure, and the top is provided with a rectangular lower groove 6; the bottom of the lower groove 6 is provided with a reflector 9, and the reflector 9 is aluminum membrane. The quartz tube 3 has an inner diameter of 3 mm and an outer diameter of 5 mm, and is clamped between the upper groove 4 and the lower groove 6 a...

Embodiment 2

[0042] For the fluorescence detection cell described in Example 1, the excitation optical path is changed to an excitation optical fiber with a core diameter of 5 mm, the excitation optical fiber is inserted into the light hole 5, and the emitted light is irradiated onto the quartz tube 3 through the excitation optical fiber. The fluorescence collection optical path is changed to a 3mm fluorescence collection optical fiber; the fluorescence collection optical fiber is inserted into the gap between the upper clamp 1 and the lower clamp 2, and its axis is perpendicular to the axis of the quartz tube 3; both the excitation optical fiber and the fluorescence collection optical fiber are physically connected to the quartz tube touch.

[0043] The experimental results are: Compared with the existing close-fitting detection pool (Talanta, 2012, 88, 463-467), the detection signal-to-noise ratio is increased by 2.5 times.

Embodiment 3

[0045] Fluorescence detection cell as described in Example 1, wherein the quartz tube 3 is changed to a quartz capillary with an inner diameter of 50 microns and an outer diameter of 365 microns; the width of the upper groove 4 and the lower groove 6 is 0.2 mm, and the depth is 0.1 mm.

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Abstract

The invention provides a flow-through fluorescence detection cell. The flow-through fluorescence detection cell comprises an upper clamping member, a lower clamping member and a quartz tube. The upper clamping member has a cylindrical structure, the lower end surface of the upper clamping member is provided with a through rectangular upper groove, and the upper end surface of the upper clamping member is downward vertically provided with a light through hole; the top of the lower clamping member is provided with a through rectangular lower groove; and the quartz tube is clamped between the upper groove and the lower groove as a flow-through cell, and the through directions of the upper groove and the lower groove parallel to the axial direction of the quartz tube. Light from a light source goes through the through hole and irradiates to the quartz tube, and strongly reflected excitation light generated by an air-quartz tube external surface optical interface is blocked by two inner side edges of the upper groove, and cannot be propagated to a fluorescence collection light path, so the high background noise caused by reflected excitation light is effectively weakened, and the detection signal-to-noise ratio is improved. The detection cell has the advantages of simple structure and easy manufacturing, and is suitable for capillary electrophoresis, liquid chromatography and flow analysis fluorescence detectors.

Description

technical field [0001] The patent of the present invention relates to the technical field of fluorescence detectors, more specifically, to a flow-through fluorescence detection cell. Background technique [0002] Liquid chromatography and capillary electrophoresis, as high-efficiency separation techniques, are widely used in the separation and analysis of mixed substances. Fluorescence detector (FLD) is a highly sensitive and highly selective detector, combined with liquid chromatography, capillary electrophoresis and fluorescence detector, widely used to detect complex mixed trace biological and environmental substances. The detection cell is one of the core components of the fluorescence detector, and the design of the detection cell directly affects the performance of the entire detection system. Researchers usually design the detection cell according to the designed excitation and emission light path structure. There are generally three ways to focus or collect the exc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/01
Inventor 关亚风耿旭辉刘洪鹏完玲中
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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