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A SNP marker related to auxiliary diagnosis of primary hepatocellular carcinoma and its application

A technology for auxiliary diagnosis and markers, applied in the field of SNP markers

Inactive Publication Date: 2017-10-20
SHANGHAI ROSETTA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there is no report on the application of SNP in the diagnosis of liver cancer. If the SNPs that are susceptible to liver cancer can be screened out as biomarkers, and the corresponding diagnostic kits can be developed, it will be a powerful impetus to the current situation of liver cancer diagnosis in my country. Its drug screening, drug efficacy evaluation and targeted therapy have opened up new avenues

Method used

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  • A SNP marker related to auxiliary diagnosis of primary hepatocellular carcinoma and its application
  • A SNP marker related to auxiliary diagnosis of primary hepatocellular carcinoma and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1 The collection of samples and the arrangement of sample data

[0065] The inventor collected a large number of blood samples from patients with liver cancer from Jiangsu and its surrounding areas from 2004 to 2013. After sorting out the sample data, the inventor selected 7323 samples that met the following criteria: whole genome chip scan and single SNP Experimental samples for Sequenom MassARRAY genotyping:

[0066] 1. Liver cancer patients with definite pathological diagnosis;

[0067] 2. Healthy controls matched with the age and sex of the cases;

[0068] The demographic data and clinical data of these samples were collected systematically.

Embodiment 2

[0069] Example 2 Whole Genome Scanning of SNPs in Peripheral Blood DNA

[0070] Among the above-mentioned 1538 eligible liver cancer patients and 1465 healthy controls, the two groups were matched in age and gender. The two groups of people were detected by Human OmniExpress12v1 chip to obtain relevant results. The specific steps are:

[0071] 1. Add the hemolysis reagent to the leukocytes stored in the 2ml cryopreservation tube, mix it upside down and transfer it completely.

[0072] 2. Removal of red blood cells: Fill the 5ml centrifuge tube to 4ml with hemolysis reagent, mix by inverting, centrifuge at 4000rpm for 10 minutes, and discard the supernatant. Add 4ml of hemolysis reagent to the precipitate, invert and wash again, centrifuge at 4000rpm for 10 minutes, and discard the supernatant.

[0073] 3. Extract DNA: add 1ml extract solution to the precipitate (each 300ml contains 122.5ml 0.2M sodium chloride, 14.4ml 0.5M ethylenediaminetetraacetic acid, 15ml 10% sodium do...

Embodiment 3

[0082] Example 3 Sequenom MassARRAY genotyping of a single SNP

[0083] The SNPs found to be associated with the onset of liver cancer in the above genome-wide scan were detected in another 2112 liver cancer cases and 2208 healthy controls. The specific steps are as follows:

[0084] 1. Add hemolysis reagent (i.e. lysate, 40 parts) to the peripheral blood stored in the 2ml cryopreservation tube. Dilute the TrisHcl solution to 2000ml, the same below), turn it upside down and mix it completely.

[0085] 2. Removal of red blood cells: Fill the 5ml centrifuge tube to 4ml with hemolysis reagent, mix by inverting, centrifuge at 4000rpm for 10 minutes, and discard the supernatant. Add 4ml of hemolysis reagent to the precipitate, invert and wash again, centrifuge at 4000rpm for 10 minutes, and discard the supernatant.

[0086] 3. Extract DNA: add 1ml extract solution to the precipitate (each 300ml contains 122.5ml 0.2M sodium chloride, 14.4ml 0.5M ethylenediaminetetraacetic acid, 15...

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Abstract

The invention belongs to the genetic engineering and tumour medicine fields and discloses an SNP marker related to primary hepatocellular carcinoma auxiliary diagnosis and an application of the SNP marker. The marker is a combination of rs7574865, rs1012068, rs17401966, rs2596542, rs455804, rs9272105, rs9275319 and rs9275572. The marker can be used for preparing a hepatic carcinoma auxiliary diagnosis kit.

Description

technical field [0001] The invention belongs to the fields of genetic engineering and tumor medicine, and relates to a SNP marker related to the auxiliary diagnosis of primary hepatocellular carcinoma and its application. Background technique [0002] Primary liver cancer is one of the most common malignant tumors in the world, and hepatocellular carcinoma (Hepatocellular Carcinoma, HCC) accounts for about 85% of it. According to an estimate by the International Agency for Research on Cancer (IARC) in 2012, there were 14.1 million cancer cases worldwide, of which 782,500 were new cases of liver cancer and 745,500 deaths, 50% of which occurred in China. According to the analysis report of national cancer incidence and death data in 2011 collected by the National Cancer Center of China in 2014, there are about 355,600 new cases of liver cancer in China, ranking fourth among all malignant tumors in my country; about 322,400 deaths, ranking first among all Cancer deaths rank sec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/156
Inventor 戴俊程江玥王一雄李绵
Owner SHANGHAI ROSETTA BIOTECH CO LTD
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