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Preparation method of conjugated glutamine for improving intestinal nutrition of piglets

A technology of glutamine and conjugated state, applied in the field of conjugated glutamine, can solve the problems of different enzymatic hydrolysis sequences, different properties and functions of enzymatic hydrolysis products, etc., and achieve the effects of high product activity, easy operation and wide source.

Active Publication Date: 2018-02-02
郑州新威营养技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Although the above research is the enzymatic hydrolysis of gluten or wheat protein, due to the different hydrolysis characteristics and enzymatic hydrolysis sequences of various proteases, the properties of the enzymatic hydrolysis products There is also a big difference in function
However, there are few researches whose main research goal is to improve the intestinal nutrition of piglets. It is only found in Liu Wenhao's "Preparation of Glutamine Peptides from Glutenin and Research on the Effects of Intestinal Nutrition" (Huazhong Agricultural University, 2008), but this literature only After the gliadin in wheat gluten is removed, the glutenin is used as raw material, and then hydrolyzed with trypsin and alkaline protease, and the present invention uses gluten as raw material, and is hydrolyzed in sequence by amylase and protease fundamentally different approach

Method used

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  • Preparation method of conjugated glutamine for improving intestinal nutrition of piglets
  • Preparation method of conjugated glutamine for improving intestinal nutrition of piglets
  • Preparation method of conjugated glutamine for improving intestinal nutrition of piglets

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Embodiment 1

[0029] Embodiment 1 The preparation method of the bound glutamine for improving intestinal nutrition of piglets comprises the following steps:

[0030]Mix gluten powder and hot water at 70°C in a weight ratio of 1:3, keep warm in the reactor for 45 minutes, then add medium-temperature α-amylase at 0.15wt% of the weight of the current material (gluten powder + hot water), React for 1.5 hours; cool the material to 50°C, adjust the pH to 9.0 with 1mol / L sodium hydroxide solution, and then press the total weight of the current material (wheat gluten + hot water + medium temperature α-amylase + sodium hydroxide solution) Add alkaline protease and papain (the weight ratio of the two is 1:1) at 1.0wt%, and react for 2.0 hours; + papain) 0.8wt% of the total weight was added to the compound protease, and the reaction was carried out for 1.5 hours under stirring; the temperature of the material was adjusted to 37°C, and the pH was adjusted to 7.8, according to the current material (whea...

Embodiment 2

[0033] Embodiment 2 is used to improve the preparation method of the combined state glutamine of piglet intestinal nutrition, comprises the steps:

[0034] Mix gluten powder and 65°C hot water in the reaction kettle at a weight ratio of 1:2 for 15 minutes, then add fungal α-amylase at 0.1% of the current material weight, and react for 0.5 hours; after the material is lowered to 55°C , adjust the pH to 8.0 with 0.5mol / L sodium hydroxide solution, then add alkaline protease and papain (the weight ratio of the two is 1:1) according to 1.2% of the current material weight, and react for 1.0 hour; then press the current material weight Add 1.0% of the compound protease and react for 1.0 hours; adjust the material temperature to 35°C, adjust the pH to 7.5, add trypsin according to 0.05% of the current material weight, and stir for 2.0 hours; use 0.5mol / L hydrochloric acid to adjust the pH to 4.3 ~4.4, heat to 100°C to inactivate the enzyme for 15 minutes, then centrifuge at 2500 rpm ...

Embodiment 3

[0037] Embodiment 3 is used to improve the preparation method of the combined state glutamine of piglet intestinal nutrition, comprises the steps:

[0038] Mix gluten powder and 75°C hot water in the reaction kettle at a weight ratio of 1:5 for 50 minutes, then add medium-temperature α-amylase at 0.3% of the current material weight, and react for 2 hours; cool the material to 55°C , adjust the pH to 10.0 with 2mol / L sodium hydroxide solution, then add alkaline protease and papain (the weight ratio of the two is 1:1) according to 0.2% of the current material weight, and react for 2.5 hours; % Add compound protease and react for 2.5 hours; adjust the temperature of the material to 38°C, adjust the pH to 8.0, add trypsin according to 0.3% of the current material weight, and react for 3.5 hours; adjust the pH to 4.8 with 2mol / L hydrochloric acid, and heat to 100 The enzyme was inactivated at ℃ for 20 minutes, and then the precipitate was removed by centrifugation at 3000 rpm; the ...

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Abstract

The invention relates to a method for preparing bound glutamine for improving intestinal nutrition of piglets. Wheat gluten is used as a raw material, and after being treated with amylase, alkaline protease / papain, compound protease, Trypsin hydrolysis, the enzymatic hydrolyzate is acidified, then heated to inactivate the enzyme, centrifuged and precipitated, the supernatant is filtered through a microfiltration membrane, concentrated under reduced pressure and spray-dried to obtain active peptides rich in bound glutamine. Animal experiments show that the product can significantly promote the growth and development of small intestinal chorion of piglets and improve intestinal nutrition of piglets. The invention has the characteristics of wide source of raw materials, easy operation, high product activity, large market consumption and the like.

Description

technical field [0001] The invention belongs to the technical field of immune regulation of piglets, and in particular relates to a method for preparing bound glutamine capable of significantly improving intestinal nutrition of piglets by using gluten as a raw material through multi-enzyme hydrolysis. Background technique [0002] The intestine is the main place for animals to digest and absorb nutrients. Intestinal development of piglets, especially weaned piglets, is not perfect, and they are sensitive to changes in external factors. The huge stress brought about by weaning and the change of feed form will damage the intestinal mucosa, resulting in a decrease in the digestion and absorption rate of nutrients and a decline in immune function. , which affects the growth and development of piglets, and then affects the health and development of the entire growth stage in the future. Therefore, it is of great significance to improve the intestinal nutrition of piglets. [00...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/14A23K20/142A23K50/30
Inventor 王章存王许东张新武朱芳周张子峰
Owner 郑州新威营养技术有限公司
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