Method for rapidly establishing medium-sized Chinese pennisetum herb tissue culture regeneration system by taking seeds as explants

Abstract

The invention provides a method for rapidly establishing a medium-sized Chinese pennisetum herb tissue culture regeneration system by taking seeds as explants. The method mainly comprises the following steps: performing selection, disinfection and soaking on a medium-sized Chinese pennisetum herb raw material; performing inoculation, culture and differentiation on callus tissues; performing rooting culture on tissue culture seedlings; and performing acclimatization and transplanting. By adopting the method provided by the invention, an indoor rapid tissue culture regeneration system of medium-sized Chinese pennisetum herb wild species can be rapidly established, and the indoor rapid tissue culture regeneration system is an important foundation for further researching a molecular mechanism with low seed setting and germination rate; and moreover, the method is simple to operate and easy to complete.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a method for quickly establishing a medium-sized pennisetum tissue culture regeneration system using seeds as explants. Background technique [0002] Medium Pennisetum ( Pennisetum longissimum var.intermedium) is a perennial wild herbaceous plant of Pennisetum genus Poaceae, which is distributed in Yunnan, Sichuan, Guizhou, Hunan and other places. The medium-sized pennisetum has thicker stems, well-developed root system, wide adaptability, strong regeneration ability, high grass yield, relatively high nutritional content, and it has strong disease resistance, drought resistance, humidity resistance and salt-alkali tolerance. It is an important species for the promotion of high-quality forage and for the study of important mechanisms such as salt tolerance, drought resistance, and high yield of gramineous plants. However, due to its low seed setting rate and germination rate in t...

AI Technical Summary

Problems solved by technology

Although the rapid propagation of Pennisetum tissue can be carried out indoors, the method of tissue culture and propagation is time-consuming and laborious, and cannot fundamentally solve the problem of large-scale propagation of medium-sized Pennisetum. The problem is to improve its seed firmness and germination rate. the key to

However, the molecular mechanism of its seed setting and low germination rate has not been studied clearly at present. The important problem is the basic conditions for its molecular mechanism research---the tissue culture regeneration system of Pennisetum with seeds as explants has not yet been established. Established, to a large extent, limited the development of relevant research, and the callus and differentiation process is relatively slow in the traditional petri dish sealed culture mode, and there is currently a lack of a medium-sized Pennisetum tissue that uses seeds as explants to quickly establish Methods for cultivating regenerative systems

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