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Urine test kit for cervical cancer

A detection kit and technology for cervical cancer, which is applied in the field of cervical cancer urine detection kits, can solve problems such as general home operations that are not applicable to patients, health risks for operators, etc.

Inactive Publication Date: 2016-06-22
HANGZHOU XIN YE BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Both Chinese patents CN202092960U and CN104181155A disclose kits for detecting mercapto groups, but both of these documents use mercuric dichloride (ie, mercuric chloride), which has serious health hazards, and has certain risks to the health of operators
CN104198404A discloses a rapid quantitative detection kit for cervical cancer screening, in which DTNB is used as a chromogenic agent, but DTNB is used to detect sulfhydryl groups in proteins and polypeptides, and this method requires the use of a biochemical instrument for detection, which is not suitable for patients. General household operations

Method used

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Examples

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Embodiment Construction

[0013] Embodiments of the present invention are described below through examples, and those skilled in the art should recognize that these specific examples only show the technical solutions selected for implementation in order to achieve the object of the present invention, and are not limitations on the technical solutions. According to the teaching of the present invention, it is obvious to improve the technical solution of the present invention in combination with the prior art, and all belong to the protection scope of the present invention.

[0014] A urine detection kit for cervical cancer comprises a container 1 and a container 2, wherein the container 1 contains 2ml of DTNB solution, and the container 2 contains 2ml of phosphotungstic acid solution.

[0015] Wherein the DTNB solution is prepared as follows: First prepare Tris-glycine buffer solution, containing 1.04g Tris, 0.69g glycine, 0.12g EDTA, pH=8.0 in every 100ml solution, and 0.4g DTNB (that is, 5,5'-dithiobis...

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PUM

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Abstract

The invention discloses a urine detection kit for cervical cancer. The urine detection kit comprises a container 1 and a container 2, wherein the container 1 is used for accommodating 2 milliliters of DTNB solution, and the container 2 is used for accommodating 2 milliliters of phosphotungstic acid solution. The invention further discloses a using method of the kit. The using method comprises the following steps: receiving 1 milliliter of first morning urine of a subject, adding dropwise the first morning urine into the container with the DTNB solution, leaving the mixture to stand for 2 to 5 minutes, then adding the mixture into the container with the phosphotungstic acid solution, leaving the mixed solution to stand for 2 to 5 minutes, and observing whether the color of the mixed solution changes or not. According to the urine detection kit and the using method, whether the subject suffers from cervical cancer or not can be conveniently and rapidly judged by color development reaction.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic reagents, in particular to a urine detection kit for cervical cancer and a use method thereof. Background technique [0002] Early detection is the key to the treatment of tumors. The development of tumors is divided into five stages: benign, tumor cell initiation, early tumor, mid-tumor, and late stage. The frightening thing about cancer is that the first three stages cannot be detected by imaging. Even in the middle stage of the tumor, only very professional doctors can detect it with advanced equipment. According to the research of the World Health Organization: 1 / 3 of cancers can be prevented at present; 1 / 3 of cancers can prolong the life of patients through active treatment; 1 / 3 of cancers can be cured through early detection, early diagnosis and early treatment of. [0003] Cervical cancer is the most common gynecological malignancy. The high-incidence age of carcinoma in situ is 30-35...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/57411
Inventor 朱建华
Owner HANGZHOU XIN YE BIOLOGICAL TECH
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