Method for constructing serum metabonomics analysis model

Abstract

The invention discloses a method for constructing a serum metabonomics analysis model. The construction method comprises the following steps: collecting a healthy serum sample and an ill serum sample; performing LC-MS detection on the samples, thereby obtaining original metabolic fingerprints; preprocessing the fingerprints, sequentially performing principal component analysis and partial least square discriminant analysis on a two-dimensional matrix, thereby obtaining a PLS-DA model; and verifying the obtained PLS-DA model, wherein if the overfitting risk does not exist, the model construction is finished. According to the method disclosed by the invention, the serum metabonomics analysis technology is applied to early screening of an esophagus cancer, the high-risk population of the esophagus cancer can be rapidly and conveniently screened by virtue of model construction, the esophagus cancer screening range is widened, the entire population screening efficiency in the high incidence area of esophagus cancer is effectively improved, the screening cost is greatly reduced, pain of partial population caused by an invasive gastroscope is effectively avoided, and the method has significant economic and social benefits and is worthy of popularization and application.

Description

technical field [0001] The invention relates to a method for constructing a serum metabolomics analysis model. The model obtained by the method of the invention can conveniently and quickly carry out early preliminary screening for esophageal cancer, and belongs to the field of analysis technology. Background technique [0002] Esophageal cancer is a malignant lesion formed by abnormal proliferation of esophageal squamous epithelium or glandular epithelium, and it is a gradual evolution process from atypical hyperplasia to carcinoma. According to the latest data from the World Health Organization, about 400,000 people die from esophageal cancer every year in the world. my country is the country with the highest incidence and mortality rate of esophageal cancer, and 90% of the patients have squamous cell carcinoma. The Wenhe River Basin in Shandong Province is a high-incidence area for esophageal cancer, with Feicheng City, Ningyang County, and Wenshang County having the high...

AI Technical Summary

Problems solved by technology

[0007] In view of the deficiencies in the prior art, such as cumbersome early screening methods for esophageal cancer, high cost, and pain to the testing population, there is no such defect that is simple, fast, and suitable for a wide range. The present invention provides a serum metabolomics analysis model. The construction method and the obtained model, the construction method of the analysis model is simple, and can replace the iodine staining indicative biopsy technique under the gastroscope for early and preliminary screening of esophageal cancer in the crowd, which is economical, convenient and quick, and can avoid the pain of the crowd to be tested. Easy to promote and apply

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