A method for constructing a serum metabolomics analysis model for primary screening of esophageal cancer

Abstract

The invention discloses a serum metabonomics analysis model. A method for constructing the serum metabonomics analysis model comprises the following steps: collecting a healthy serum sample and a sick serum sample; carrying out LC-MS detection on the samples to obtain an original metabonomics fingerprint spectrum; preprocessing the spectrum, sequentially carrying out principal component analysis and partial least square discriminant analysis on the obtained two-dimensional matrix so as to obtain a PLS-DA model, and verifying the obtained PLS-DA model, and constructing the model if no overfitting risk exists. The serum metabonomics analysis model has the advantages that a serum metabonomics analysis technology is initially applied to early esophagus cancer screening, high-risk esophagus cancer population can be rapidly and conveniently screened out by utilizing the serum metabonomics analysis model, an esophagus cancer screening range is narrowed, the efficiency of screening high-risk esophagus cancer population is effectively improved, the screening cost is greatly reduced, pain, caused by a gastroscope, on part of population is effectively avoided, and important economic benefit and social benefit are realized, so that the serum metabonomics analysis model is convenient for popularization and application.

Description

technical field [0001] The invention relates to a serum metabolomics analysis model, which can conveniently and quickly carry out early preliminary screening for esophagus cancer, and belongs to the field of analysis technology. Background technique [0002] Esophageal cancer is a malignant lesion formed by abnormal proliferation of esophageal squamous epithelium or glandular epithelium, and it is a gradual evolution process from atypical hyperplasia to carcinoma. According to the latest data from the World Health Organization, about 400,000 people die from esophageal cancer every year in the world. my country is the country with the highest incidence and mortality rate of esophageal cancer, and 90% of the patients have squamous cell carcinoma. The Wenhe River Basin in Shandong Province is a high-incidence area for esophageal cancer, with Feicheng City, Ningyang County, and Wenshang County having the highest incidence rates of 93.95 / 100,000, 88.68 / 100,000, and 62.26 / 100,000,...

AI Technical Summary

Problems solved by technology

[0007] In view of the deficiencies in the prior art, such as cumbersome early screening methods for esophageal cancer, high cost, and pain to the testing population, there is no such defect that is simple, fast, and suitable for a wide range. The present invention provides a serum metabolomics analysis model, The construction method of the analysis model is simple, and it can replace the iodine-stained indicative biopsy technique under the gastroscope for early and preliminary screening of esophageal cancer in the population.

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