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Method for simultaneously detecting multiple sugars in coffee

A coffee and retention time technology, applied in the field of ion chromatography detection, can solve the problems of difficulty in separating small molecule oligosaccharides, mutual interference between sugar xylose and mannose, non-specificity, etc., achieving reliable results, solving false positives, Accurate results

Active Publication Date: 2015-06-17
QINGDAO INSPECTION & QUARANTINE TECH DEV CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the determination methods of sugar in coffee products are mainly ion chromatography using amperometric detector and high performance liquid chromatography using photodetector. The absorption wavelengths are the same under conditions such as detectors, the two types of detectors are not specific, and there are great difficulties in the separation of small molecule oligosaccharides
At present, the analysis of sugar in coffee products in the AOAC standard has the problem of mutual interference between xylose and mannose

Method used

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  • Method for simultaneously detecting multiple sugars in coffee
  • Method for simultaneously detecting multiple sugars in coffee
  • Method for simultaneously detecting multiple sugars in coffee

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The method for simultaneously detecting multiple sugars in coffee provided by the invention comprises the following steps:

[0029] (1) Sample pretreatment

[0030] Take a coffee bean sample and crush it on a grinder. Take 0.5g of the crushed sample, add 49.5g of deionized water, shake it well at 30 degrees Celsius for 30 minutes, centrifuge at 5000r / min for 5 minutes, and take the upper layer to pass through a 0.22um microporous filter overnight. Membrane filtration, filtered solution injection analysis;

[0031] (2) Sample injection analysis

[0032] The sample was separated by ion chromatography, the mobile phase was 5mM NaOH solution, and the chromatographic column was Dionex CarboPac TM PA20 chromatographic column (3*150mm, Thermo), equipped with Dionex CarboPac TM PA20 guard column (3*30mm, Thermo);

[0033] (3) Qualitative and quantitative analysis

[0034] The standard solutions of various sugars were injected and analyzed according to the above step...

Embodiment 2

[0040] Embodiment 2 Application example

[0041] Analyze and measure KIMBO ESPRESSO ITALIAND coffee samples purchased in the market to test which sugars are included.

[0042] (1) Sample pretreatment

[0043] Take 100g of coffee sample and crush it again on a grinder. Take 0.5g of the crushed sample, add 49.5g of deionized water at a temperature of 80 degrees Celsius, oscillate and shake for 30min, cool and centrifuge at 5000r / min for 5min, and take the upper layer to clear overnight for 0.22 um microporous membrane filtration, and the filtered solution was sampled for analysis.

[0044] (2) Sample injection analysis

[0045] The sample was separated by ion chromatography, the mobile phase was 5mM NaOH solution, and the chromatographic column was Dionex CarboPac TMPA20 chromatographic column (3*150mm, Thermo), equipped with Dionex CarboPac TM PA20 guard column (3*30mm, Thermo);

[0046] (3) Qualitative and quantitative analysis

[0047] Inject and analyze the stand...

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Abstract

The invention discloses a method for simultaneously detecting multiple sugars in coffee. The method comprises the following steps: (1) performing pretreatment on a sample; (2) feeding the sample to analyze; (3) performing qualitative and quantitative analysis. The method can be adopted to simultaneously detect multiple sugars in coffee, and is simple to operate and accurate and reliable in result.

Description

technical field [0001] The invention relates to a method for simultaneously detecting multiple sugars in coffee, belonging to the field of ion chromatography detection. Background technique [0002] In view of the different drinking objects of coffee products and the quality supervision of coffee products, the sugar content in coffee products needs to be strictly tested. At present, the determination methods of sugar in coffee products are mainly ion chromatography using amperometric detector and high performance liquid chromatography using photodetector. The absorption wavelengths of the detectors and other conditions are the same, and the two types of detectors are not specific, and there are great difficulties in the separation of small molecule oligosaccharides. At present, the analysis of sugar in coffee products in the AOAC standard has the problem of mutual interference between xylose and mannose. In this research method, ion chromatography is used to preliminarily ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 崔鹤马甲民张辉珍彭云霞刘静静张文皓
Owner QINGDAO INSPECTION & QUARANTINE TECH DEV CENT
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