Establishing method and application of mouse aortic dissecting aneurysm model

Abstract

The invention discloses an establishing method and application of a mouse aortic dissecting aneurysm model. The establishing method of the mouse aortic dissecting aneurysm model is simple and convenient, the mouse aortic dissecting aneurysm model can be established within a short time, surgical operation is not needed, trauma to experimental mice can be reduced, and high operability is achieved. The occurrence rate of aortic dissecting aneurysm of a modeling group is 90%, the breaking rate of the aortic dissecting aneurysm of the modeling group is 20%, the occurrence rate of the aortic dissecting aneurysm in chests and aortic dissection above chests is 90%, the occurrence rate of the aortic dissecting aneurysm in abdomen is 20%, and the induction success rate is high. The dissection occurrence position of the aortic dissecting aneurysm is similar to that of human beings, so that the mouse aortic dissecting aneurysm model can be used for easily studying the pathomechanism of the aortic dissecting aneurysm of human beings and can be applied to suspicious virulence gene screening and treatment research.

Description

technical field [0001] The invention relates to a method for establishing a mouse aortic dissection aneurysm model and its application in the technical field of making animal models of human diseases. Background technique [0002] Aortic dissecting aneurysm refers to the rupture of intima vessels in the aortic wall caused by various reasons, and between the intima and the outer and middle layers, due to the pressure of blood hydrodynamics, it is stripped longitudinally along this layer, forming an active blood false lumen. Due to dissection separation, the false lumen expanded and bulged. The resulting dissecting aneurysm is highly susceptible to rupture, making the disease one of the deadliest forms of cardiovascular disease, with a mortality rate of up to 70% within two weeks of onset. [0003] Aortic aneurysms occur more frequently in the West, mainly in the abdominal aorta, while aortic dissection is more common in China, mostly involving the aortic root, ascending aort...

AI Technical Summary

Problems solved by technology

However, the establishment of models often requires specific gene knockout animals or drug treatment through surgery. The hybridization of experimental animals takes a long time, and the surgery requires high technical requirements for experimenters, making it difficult to establish models.

The establishment of an aortic dissection model in mice basically requires mutations in certain genes in the mouse, and the function is affected, such as Fbn1, etc.; there are few reports on the establishment of aortic dissection in normal wild-type mice, which limits its use. Research on pathogenesis and treatment methods

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