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Avian infectious bronchitis virus attenuated virus strain rH120-YZ and construction method thereof

A rh120-yz, bronchitis technology, applied in the field of vaccine manufacturing, can solve the problems of genotype inconsistency, etc., and achieve the effects of low pathogenicity, high reproductive titer, and broad application prospects

Inactive Publication Date: 2015-06-10
JIANGSU INST OF POULTRY SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The first purpose of the present invention is to invent a weakened strain of chicken infectious bronchitis virus, thereby solving the problem that the genotypes of the currently used IB vaccine strains are inconsistent with those of the epidemic strains

Method used

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  • Avian infectious bronchitis virus attenuated virus strain rH120-YZ and construction method thereof
  • Avian infectious bronchitis virus attenuated virus strain rH120-YZ and construction method thereof
  • Avian infectious bronchitis virus attenuated virus strain rH120-YZ and construction method thereof

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Embodiment Construction

[0024] The following examples describe the present invention in detail, and the examples are only intended to illustrate the present invention, but are not intended to limit the scope of the present invention. The scope of the invention is specifically defined by the appended claims.

[0025] Step 1: Cloning of Spike Protein Gene S of Infectious Bronchitis Virus Isolate

[0026] Preparation of biological material:

[0027] Infectious bronchitis virus isolate ck / CH / IBYZ / 2011 was isolated, identified and preserved by the Institute of Poultry Disease Control, Jiangsu Institute of Poultry Science. The applicant commits to release to the public for 20 years from the filing date.

[0028] SPF chicken embryos: purchased from Beijing Merial Verton Experimental Animal Technology Co., Ltd.

[0029] pMD18T vector: purchased from Dalian Bao Biological Engineering Co., Ltd.

[0030] JM109 genetically engineered bacteria: purchased from Dalian Bao Biological Engineering Co., Ltd.

[...

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Abstract

The invention relates to an avian infectious bronchitis virus attenuated virus strain rH120-YZ and a construction method thereof. The microbial preservation number of the avian infectious bronchitis virus attenuated virus strain rH120-YZ is CGMCCNo. 8501. The invention relates to an application of a reverse genetic manipulation technology. By using a constructed reverse genetic manipulation platform of the avian infectious bronchitis virus attenuated virus strain rH120-YZ and adopting a spike protein gene S of an avian infectious bronchitis virus attenuated virus isolate ck / CH / IBYZ / 2011 to replace a corresponding part of the H120 vaccine strain genome, a recombinant virus rH120-YZ is saved and obtained. The virus has relatively low pathogenicity on chicks, has a relatively high breeding titer on chick embryos, is suitable for large-scaled production of infectious bronchitis vaccines and can be used for manufacturing vaccines.

Description

technical field [0001] The invention relates to applying reverse genetic manipulation technology to produce rH120-YZ attenuated strain of chicken infectious bronchitis virus for making vaccine. Background technique [0002] Infectious bronchitis (IB), one of the second-class infectious diseases of poultry stipulated by the International Veterinary Bureau, is an acute, highly contagious infectious disease caused by infectious bronchitis virus (IBV) , causing serious economic losses to the poultry industry, seriously restricting the development of the poultry industry in the world [Cavanagh D. Coronaviruses in poultry and other birds. Avian Pathol, 2005, 34(6): 439-448.]. Although vaccines play an important role in disease control, with the continuous emergence of different lesion types and tissue-tropic strains, vaccines developed by traditional methods are far from meeting the needs of IB prevention and control. Based on this, developing safe and efficient new vaccines has ...

Claims

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Application Information

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IPC IPC(8): C12N7/01C12N15/09C12N15/50C12R1/93
Inventor 周生邹剑敏戴有理戴亚斌唐梦君陈连颐许明姜逸赵宝华程旭沈欣悦李建梅尤素兰
Owner JIANGSU INST OF POULTRY SCI
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