Efficient elderberry cultivation method
A cultivation method and technology of elderberry, applied in the field of high-efficiency cultivation of elderberry, can solve the problems of low survival rate and preservation rate, and achieve the effects of improving the output value per mu, improving the quality of flowers, and improving the quality of elderberry.
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Embodiment 1
[0017] (1) Cutting treatment: obtain high-quality cutting seedlings with a root number equal to or greater than 5. The cuttings must be lignified and not infected with diseases and insect pests. Leave 3 to 5 nodes and remove all leaves; the diameter of the cuttings is ≥0.5 cm. Dip in 30 to 200 mg / L growth regulator for 5 seconds to 2 minutes, cut the treated cuttings, and obtain high-quality cutting seedlings with roots equal to or greater than 5;
[0018] (2) Prepared substrate: It is composed of the following ingredients by weight: 30-50 parts of fully fermented rice bran, 50-70 parts of humus soil, 6-10 parts of bio-organic fertilizer, 5-8 parts of garden soil, bacterium 0.3-0.6 parts of clear water, 3-5 parts of perlite and 3-5 parts of vermiculite, dilute formalin with water 40 to 80 times and then spray the substrate, use 45m1 of formalin per square meter of soil; apply magnetic field to cuttings Magnetization treatment, specifically: placing the cuttings under the condi...
Embodiment 2
[0023] The mensuration of chlorogenic acid content in the elderberry of embodiment 2
[0024] Accurately weigh 0.5 g of Mengjin No. 1 elderberry sample (passed through No. 4 sieve), put it in a stoppered Erlenmeyer flask, accurately add 50 ml of 50% methanol, weigh it, ultrasonically treat it for 30 minutes, let it cool, and weigh it again. Use 50% methanol to make up for the lost weight, shake well, filter, accurately measure 5ml of the filtrate, put it in a 25ml brown measuring bottle, add 50% methanol to the mark, and set aside.
[0025] Accurately weigh 0.0041 g of the chlorogenic acid reference substance into a 100 ml brown volumetric flask, add 50% methanol to dissolve to a constant volume, and obtain a reference substance solution with a concentration of 41 μg / ml.
[0026] Accurately draw 5 μl each of the reference substance solution and the test solution, inject it into the liquid chromatograph, and measure it. The chromatographic conditions are: determined by methodo...
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