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A fermentation medium for producing glutamic acid by fermentation of thermosensitive strains and a fermentation method and application thereof for producing glutamic acid

A fermentation medium, glutamic acid technology, applied in the direction of fermentation, can solve the problems of reducing production efficiency, increasing the preparation time of fermentation medium, slow dissolving phosphate, etc., to achieve the effect of improving yield

Active Publication Date: 2019-02-15
COFCO BIO CHEM ENERGY LONGJIANG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In actual production and operation engineering, the dissolution rate of phosphate is relatively slow, and it takes a long time for phosphate to completely dissolve in water, which may easily cause uneven mixing of the fermentation medium, increase the preparation time of the fermentation medium, and reduce production. efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A fermentation medium for producing glutamic acid by fermenting thermosensitive strains, the raw material ratio of the fermentation medium is as follows:

[0037] Corn starch hydrolyzed sugar 165-185g / L, corn steep liquor 18-25g / L, molasses 10-18g / L, betaine 0.5-1.5g / L, soybean meal hydrolyzate 5-10g / L, H 3 PO 4 3~8g / L, MgSO 4 ·7H 2 O 1.25~1.75g / L, MnSO 4 ·7H 2 O0.064~0.084g / L, FeSO 4 ·7H 2 O 0.055~0.085g / L, KCl 3~6g / L, threonine 0.08~0.15g / L, succinic acid 0.8-1.2g / L, defoamer 0.1~0.3g / L, solvent is water, use Sodium hydroxide neutralizes and adjusts the pH of the fermentation medium to be 6.8-7.0.

[0038] Utilize the above-mentioned fermentation medium to produce the fermentation method of glutamic acid, the processing step and the condition of its fermentation culture are as follows:

[0039] (1) Ingredients: according to the ratio, weigh all kinds of raw materials and dissolve them completely to make 6L fermentation medium;

[0040] The raw material ratio ...

Embodiment 2

[0049] A fermentation medium for producing glutamic acid by fermenting thermosensitive strains, the raw material ratio of the fermentation medium is as follows:

[0050] Corn starch hydrolyzed sugar 165-205g / L, corn steep liquor 15-30g / L, molasses 15-18g / L, betaine 1-1.5g / L, soybean meal hydrolyzate 5-10g / L, H 3 PO 4 3~8g / L, MgSO 4 ·7H 2 O 1.25~1.75g / L, MnSO 4 ·7H 2 O 0.064~0.084g / L, FeSO 4 ·7H 2 O 0.055~0.085g / L, KCl 3~6g / L, threonine 0.08~0.15g / L, succinic acid 0.8-1.2g / L, defoamer 0.1~0.3g / L, solvent is water, use Sodium carbonate neutralizes and adjusts the pH of the fermentation medium to be 6.8-7.0.

[0051] Utilize the above-mentioned fermentation medium to produce the fermentation method of glutamic acid, the processing step and the condition of its fermentation culture are as follows:

[0052] (1) Ingredients: according to the ratio, weigh all kinds of raw materials and dissolve them completely to make 6L fermentation medium;

[0053] The raw material ratio o...

Embodiment 3

[0062] A fermentation medium for producing glutamic acid by fermenting thermosensitive strains, the raw material ratio of the fermentation medium is as follows:

[0063] Corn starch hydrolyzed sugar 165-205g / L, corn steep liquor 15-30g / L, molasses 10-18g / L, betaine 0.5-1.5g / L, soybean meal hydrolyzate 8-10g / L, H 3 PO 4 5~6g / L, MgSO 4 ·7H 2 O 1.25~1.75g / L, MnSO 4 ·7H 2 O0.064~0.084g / L, FeSO 4 ·7H 2 O 0.055~0.085g / L, KCl 3~6g / L, threonine 0.08~0.15g / L, succinic acid 0.8-1.2g / L, defoamer 0.1~0.3g / L, solvent is water, use The ammonia water neutralizes and adjusts the pH of the fermentation medium to be 6.8-7.0.

[0064] Utilize the above-mentioned fermentation medium to produce the fermentation method of glutamic acid, the processing step and the condition of its fermentation culture are as follows:

[0065] (1) Ingredients: according to the ratio, weigh all kinds of raw materials and dissolve them completely to make 6L fermentation medium;

[0066]The raw material ratio ...

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Abstract

The invention relates to a fermentation medium for fermenting and producing glutamic acid from a thermo-sensitive type strain. The components and the final concentration of the fermentation medium in the formula are as follows: 165-205g / L corn starch amylolysis, 15-30g / L maize slurry, 10-18g / L molasses, 0.5-1.5g / L glycine betaine, 5-10g / L bean pulp hydrolysate, 3-8g / L H3PO4, 1.25-1.75g / L MgSO4.7H2O, 0.064-0.084g / L MnSO4.7H2O, 0.055-0.085g / L FeSO4.7H2O, 3-6g / L KCl, 0.08-0.15g / L threonine, 0.8-1.2g / L succinic acid, and 0.1-0.3g / L defoamer, and the solvent is water, the pH value of the fermentation medium is 6.8-7.0 when the phosphoric acid is neutralized by alkali. Due to the appropriate carbon source, the nitrogen source, the carbon nitrogen ratio and the phosphorus content in the medium, the acid production rate is increased, the acid production of the thallus is promoted, the final acid production level is obviously improved by about 5.5% and the glutamic acid yield and the glucose acid conversion ratio are increased.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation engineering, in particular to a fermentation medium for producing glutamic acid by fermentation of thermosensitive strains and a fermentation method and application thereof for producing glutamic acid. Background technique [0002] Inorganic salts are indispensable substances for microbial life activities. Phosphorus, potassium and other nutrients are needed in the fermentation process of glutamic acid. Phosphorus is the main element of oxidative phosphorylation in the metabolic process of inorganic salts. It is also a component of nucleic acids, phospholipids, and certain coenzymes; high-energy phosphate bonds play an important role in energy storage and transmission. Phosphate is also an important buffering agent. It is no exception in glutamic acid fermentation. The level of phosphorus content has a great influence on it. If the concentration of phosphorus is too high, the meta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/14
Inventor 佟毅韩隽王晓丽李辉
Owner COFCO BIO CHEM ENERGY LONGJIANG CO LTD
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