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Method for extraction of biological fresh-keeping agent from berberis poiretii schneid and use of biological fresh-keeping agent

A biological preservative and three-needle technology, applied in application, fruit and vegetable preservation, food science, etc., can solve problems such as difficult cleaning, hidden dangers to human health, difficulties in storage and preservation, and achieve the effect of avoiding pollution

Inactive Publication Date: 2015-06-03
崔桂林
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Fruits, because of their high moisture content, delicious taste, and rich nutritional value, but generally berry fruits have high respiratory intensity, poor protective tissue, difficult storage and preservation, and short storage and shelf life, which will affect fruit farmers and consumers. bring great loss
At present, the conventional fresh-keeping method of berry fruits mainly adopts chemical fungicides, and the large-scale use of chemical fungicides will lead to drug resistance of pathogenic microorganisms and cause environmental pollution; in addition, because cleaning is difficult, it is difficult to thoroughly wash Remove chemical fungicides, which will cause human health hazards after consumption

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] A method for extracting biological preservatives from three needles, the operation steps are as follows: 1. Clean the three needles to remove soil, leaves and other sundries; 2. Crush the three needles to 50 mesh; 3. Put three needles of pulverized powder into a round bottom flask, add ethanol solution at a ratio of 1:2, install a reflux condenser, heat and reflux in a hot water bath for 0.5h, and cool and stand for 0.5h. Suction filtration, take the filtrate; 4, add the filter residue to the ethanol solution with the ratio of material to liquid as 1:1, condense and reflux again for 1h, cool and stand for 0.5h, suction filtration, take the filtrate; 5, combine the two filtrates, Ethanol solution was distilled off under reduced pressure; 6. Add 1% acetic acid solution to the extract at a volume ratio of 1:3, heat to dissolve, and filter while hot to remove insoluble matter to obtain a biological preservative.

[0011] The present invention also provides the application o...

Embodiment 2

[0013] A method for extracting biological preservatives from three needles, the operation steps are as follows: 1. Clean the three needles to remove soil, leaves and other sundries; 2. Crush the three needles to 100 mesh; 3. Put three needles of crushed powder into a round-bottomed flask, add ethanol solution at a ratio of 1:5, install a reflux condenser, heat and reflux in a hot water bath for 1h, and cool and stand for 0.5h. Filtrate and get the filtrate; 4. Add the filter residue to the ethanol solution at a ratio of 1:2 to the material-liquid ratio, condense and reflux for 1.5h again, cool and stand for 0.5h, filter with suction, and get the filtrate; Ethanol solution was distilled off under reduced pressure; 6. Add 2% acetic acid solution to the extract at a volume ratio of 1:5, heat to dissolve, and filter while hot to remove insoluble matter to obtain a biological preservative.

[0014] The present invention also provides the application of extracting biological preserv...

Embodiment 3

[0016] A method for extracting biological preservatives from three needles, the operation steps are as follows: 1. Clean the three needles to remove soil, leaves and other sundries; 2. Crush the three needles to 70 mesh; 3. Put three needles of pulverized powder into a round-bottomed flask, add ethanol solution at a ratio of 1:4, install a reflux condenser, heat and reflux in a hot water bath for 0.7h, and cool and stand for 0.5h. Suction filtration, take the filtrate; 4, add the ethanol solution to the ratio of 1:1.5 to the filter residue with the ratio of material to liquid, condense and reflux again for 1.2h, cool and stand for 0.5h, suction filtration, take the filtrate; 5, merge the two filtrates, The ethanol solution was evaporated under reduced pressure by the water pump; 6. Add 1.5% acetic acid solution to the extract at a volume ratio of 1:4, heat to dissolve, and filter while hot to remove insolubles to obtain a biological preservative.

[0017] The present invention...

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PUM

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Abstract

The invention relates to a method for extraction of a biological fresh-keeping agent from berberis poiretii schneid and a use of the biological fresh-keeping agent. The method comprises the following steps of crushing the berberis poiretii schneid to obtain powder of 50-100 meshes, putting the crushed berberis poiretii schneid into a round-bottom flask, adding ethanol into the crushed berberis poiretii schneid according to a material-liquid ratio of 1: 2-5, installing a reflux condensing tube, carrying out heating reflux in a hot bath for 0.5-1h, carrying out cooling standing for 0.5h, carrying out suction filtration, taking the filtrate, adding an ethanol solution into the filter residue according to a material-liquid ratio of 1: 1-2, carrying out reflux condensation for 1-1.5h, carrying out cooling standing for 0.5h, carrying out suction filtration, collecting the filtrate, merging the filtrate obtained by the two steps, evaporating the ethanol solution under the condition of water pump pressure reduction, adding 1-2% of an acetic acid solution into the extract according to a volume ratio of 1: 3-5, carrying out heating dissolution, and carrying out hot suction filtration to remove insoluble substances and to obtain the biological fresh-keeping agent. The method extracts the biological fresh-keeping agent from berberis poiretii schneid as a raw material and the biological fresh-keeping agent is used for fruit fresh-keeping and is safe. The biological fresh-keeping agent is nontoxic and nuisanceless, can replace a chemical bactericide and an antiseptic for fruit fresh-keeping, and prevents chemical bactericide-caused environmental pollution and food safety problems.

Description

technical field [0001] The invention relates to the field of biological preservation, in particular to a method for extracting a biological preservation agent from three needles and its application. Background technique [0002] The three needles are the pseudo-variant barberry. Mainly contain berberine (berberine), palmatine (palmatine), berbamine (berbamine), jatrorrhizine (jatrorrhizine), in addition to African tetrandrine (columbamine), spike Alkali (oxyacanthine, oxyacanthine), isotetrandine, magnoflorine, etc. The study found that (1) berbamine has a significant inhibitory effect on rat sarcoma W256, and the combination of berbamine and cyclophosphamide has an additive effect, which is stronger than both alone. Isotetradine has inhibitory effect on Ehrlich ascites tumor. Palmatine, jatrorrhizine, and spikenine inhibited the oxygen uptake of ascites cancer cells in mice. (2) Berbamine has a significant effect on increasing white blood cells. For cyclophosphamide-in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L3/3472
CPCA23B7/153
Inventor 崔桂林
Owner 崔桂林
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