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Extraction method of berberis poiretii schneid polysaccharide

An extraction method and three-needle technology, applied in the field of three-needle polysaccharide extraction, can solve the problems of three-needle polysaccharide yield impact, cumbersome operation, and low efficiency of three-needle polysaccharide extraction, so as to improve reliability, The effect of simple extraction operation and low equipment requirements

Active Publication Date: 2021-05-14
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for extracting three-needle polysaccharides, so as to solve the above-mentioned common plant polysaccharide extraction methods in the market today. Equally, it will affect the yield of three needle polysaccharides, which leads to the problem of low extraction efficiency of three needle polysaccharides

Method used

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  • Extraction method of berberis poiretii schneid polysaccharide
  • Extraction method of berberis poiretii schneid polysaccharide
  • Extraction method of berberis poiretii schneid polysaccharide

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Experimental program
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Effect test

Embodiment 1

[0050] A method for extracting three needle polysaccharides, comprising the steps of:

[0051] S1: Instrument preparation, prepare circulating water multi-purpose vacuum pump, constant temperature water bath, CNC ultrasonic cleaner, ultraviolet / visible spectrophotometer, desktop high-speed refrigerated centrifuge, electronic analytical balance and high-speed pulverizer;

[0052] S2: Prepare raw materials, prepare glucose, concentrated sulfuric acid, phenol, absolute ethanol, distilled water and three needles, and the above reagents are domestic analytical reagents;

[0053] S3: Raw material crushing: the three needle raw materials are crushed through a Chinese herbal medicine grinder;

[0054] S4: Filter residue acquisition: Weigh 500 g of three needle powders of appropriate size and place them in a round bottom flask, use a Soxhlet extractor to reflux for 2.0 hours through 2L of absolute ethanol, filter after the reflux is completed, evaporate the ethanol and keep the filter ...

Embodiment 2

[0079] A method for extracting three needle polysaccharides, comprising the steps of:

[0080] S1: Instrument preparation, prepare circulating water multi-purpose vacuum pump, constant temperature water bath, CNC ultrasonic cleaner, ultraviolet / visible spectrophotometer, desktop high-speed refrigerated centrifuge, electronic analytical balance and high-speed pulverizer;

[0081] S2: Prepare raw materials, prepare glucose, concentrated sulfuric acid, phenol, absolute ethanol, distilled water and three needles, and the above reagents are domestic analytical reagents;

[0082] S3: Raw material crushing: the three needle raw materials are crushed through a Chinese herbal medicine grinder;

[0083] S4: Filter residue acquisition: Weigh 500 g of three needle powders of appropriate size and place them in a round bottom flask, use a Soxhlet extractor to reflux for 2.0 hours through 2L of absolute ethanol, filter after the reflux is completed, evaporate the ethanol and keep the filter ...

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Abstract

The present invention discloses an extraction method of berberis poiretii schneid polysaccharide. The method comprises the following steps: preparing an instrument, preparing a circulating water multipurpose vacuum pump, a constant-temperature water bath kettle, a numerical control ultrasonic cleaner, an ultraviolet / visible spectrophotometer, a table-type high-speed refrigerated centrifuge, an electronic analytical balance and a high-speed pulverizer, preparing raw materials, and preparing glucose, concentrated sulfuric acid, phenol, absolute ethyl alcohol, distilled water and berberis poiretii schneid which are are all domestic analytically pure reagents. According to the extraction method of berberis poiretii schneid polysaccharide, on the basis of a single-factor test, the hot water extraction condition of the berberis poiretii schneid polysaccharide is optimized by adopting response surface method test design so as to determine the optimal extraction process condition of the berberis poiretii schneid polysaccharide, so that the credibility of a prediction result is favorably improved, and the optimal experiment condition of a multi-factor system can be quickly determined; and reference is provided for further development and utilization of the plant polysaccharide taking the berberis poiretii schneid as the raw material in the field of traditional Chinese medicines.

Description

technical field [0001] The invention relates to the technical field of three-needle polysaccharide extraction, in particular to a method for extracting three-needle polysaccharide. Background technique [0002] The three needles are the roots, stems and bark of various plants such as Berberidaceae. They have the functions of clearing away heat and dampness, purging fire and detoxifying. Streptococcus pneumococcus, pneumococcus, Shigella, Escherichia coli, Pseudomonas aeruginosa, Proteus, and Leptospira all have inhibitory effects. At the same time, studies have proved that plant polysaccharides are the main components of medicinal plant decoctions and have complex biological properties. Activity and function are considered to be one of the main medicinal substances for traditional Chinese medicine to play a traditional therapeutic role. In recent years, due to the complex structure and composition of polysaccharides from different plant sources, and the variety of functional...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003Y02A50/30
Inventor 候冉冉程磊李梦瑶孙俊乐李友英许志强丁城沣高飞
Owner QINGDAO AGRI UNIV
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