Fermentation method of banana-flavor beer
A fermentation method and beer technology, which is applied in the field of microbial fermentation and fermentation preparation of banana-flavored beer, can solve the problem of losing banana and other fruit flavors, and achieve the effect of lowering blood cholesterol
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Embodiment 1
[0018] [1] Preparation of seed liquid: After activating and culturing the brewer's yeast strain for 1-2 days, inoculate it from the slant lawn into a triangular flask containing 50ml of wort medium, and culture it with shaking at 30°C for 24-36 hours to cultivate well. The bacterial suspension is the seed liquid;
[0019] [2] Card fermenter fermentation: inoculate the beer yeast strain suspension into a card fermenter equipped with wort medium at an inoculum amount of 8-12vol% for cultivation, and the culture temperature of the fermenter is 28-32°C , cultivate pH5.3-5.7, take samples every 4-6 hours to detect the cell concentration, stop the fermentation after 24-48 hours of cultivation, and separate the fermentation broth to get the cells;
[0020] [3] Wort preparation: the proportion of malt in the medium is 20% (W / V), weigh and crush the malt, add tap water in portions according to the weight / volume ratio of 1:5, hydrolyze at 50°C for 70 minutes, and continue to heat up Hy...
Embodiment 2
[0026] [1] See Example 1;
[0027] [2] See Example 2;
[0028] [3] Wort preparation: the proportion of malt in the medium is 18% (W / V), weigh and crush the malt, add tap water in portions according to the weight / volume ratio of 1: 5, hydrolyze at 55°C for 70 minutes, and continue to heat up Hydrolyze at 65°C for 80 minutes, then filter the hydrolyzate, discard the solid matter, take a sample to measure the sugar, the sugar concentration should be around 12.0;
[0029] [4] Sterilization of wort: Boil the prepared wort at 100°C for 1 hour to sterilize, add bitter hops at 0.05% 5 minutes after the start of boiling, add fragrant hops at 0.03% 10 minutes before the end of boiling Add, peel the bananas, homogenize the pulp, sterilize at 60°C for 15 minutes, and then add in an amount of 16%;
[0030] [5] Fermentation tank cleaning and sterilization: first rinse the fermentation tank with tap water for 20 minutes, then rinse with lye for 20 minutes, rinse with tap water for 20 minut...
Embodiment 3
[0034] [1] See Example 1;
[0035] [2] See Example 1;
[0036] [3] Wort preparation: the proportion of malt in the medium is 16.7% (W / V), weigh and crush the malt, add tap water in portions according to the weight / volume ratio of 1: 5, hydrolyze at 60°C for 60 minutes, and continue to heat up Hydrolyze at 65°C for 80 minutes, then filter the hydrolyzate, discard the solid matter, take a sample to measure the sugar, the sugar concentration should be around 11.0;
[0037] [4] Sterilization of wort: Boil the prepared wort at 100°C for 1 hour to sterilize, add bitter hops at 0.05% 5 minutes after the start of boiling, add fragrant hops at 0.03% 10 minutes before the end of boiling To add, peel the bananas, homogenate the pulp, sterilize at 60°C for 15 minutes, and then add in an amount of 17%;
[0038] [5] Fermentation tank cleaning and sterilization: first rinse the fermentation tank with tap water for 20 minutes, then rinse with lye for 20 minutes, rinse with tap water for 20 ...
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