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Callicarpa nudiflora extract containing phenylethanoid glycoside and preparation method thereof

A technology of nude flower purple pearl and phenylethanol glycoside, which is applied in the field of natural medicine and can solve problems such as treating the symptoms but not the root cause

Active Publication Date: 2015-05-27
HAINAN MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Comprehensive treatment is often advocated now. Although it can protect liver parenchymal cells, reduce liver damage, slow down the decline of liver function, and control the disease, the existing drugs only treat the symptoms and not the root cause.

Method used

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  • Callicarpa nudiflora extract containing phenylethanoid glycoside and preparation method thereof
  • Callicarpa nudiflora extract containing phenylethanoid glycoside and preparation method thereof
  • Callicarpa nudiflora extract containing phenylethanoid glycoside and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] 1. Preparation

[0056] Extraction: Take 300g of Auranthus nudiflora leaves, add 6000mL of distilled water, soak for 2 hours, heat and extract for 1 hour, filter, add 4500mL of distilled water to the filter residue and heat and extract twice, each time for 1 hour, combine the extracts for 3 times, and pass the extracts through 300 mesh Filter through gauze.

[0057] Impurity removal: heat and concentrate the filtrate to 600mL (0.5g / mL), add chitosan clarifier according to the volume ratio of liquid medicine and clarifier 1:0.15, and stir for 30min at a temperature of 60°C at a stirring speed of 150r / min. Stand still for 2 hours, centrifuge (3000r / min, 10min).

[0058] Refining: Take the supernatant after centrifugation, add SP-207 macroporous resin according to the amount of resin and medicinal material 1:0.8, perform dynamic adsorption according to the adsorption flow rate of 2BV / h, and the ratio of diameter to height is 1:10, respectively use 3BV distilled water , 3...

Embodiment 2

[0075] Extraction: Take 300g of Auranthus nudiflora leaves, add 4500mL of distilled water, soak for 2 hours, heat and extract for 2 hours, filter, add 3000mL of distilled water to the filter residue, heat and extract for 2 hours each time, combine the extracts twice, and filter the extracts through 300 mesh gauze .

[0076] Impurity removal: The filtrate is heated and concentrated to 1200mL (0.25g / mL), and 101 fruit juice clarifier is added according to the volume ratio of medicinal liquid and clarifier 1:0.08. Set aside for 12 hours, centrifuge (3000r / min, 10min).

[0077] Refining: Take the supernatant after centrifugation, add SP-825 macroporous resin according to the amount of resin and medicinal material at 1:1.0, and perform dynamic adsorption according to the adsorption flow rate of 1BV / h, and the diameter-to-height ratio is 1:8. , 4BV 20% ethanol, 6BV 60% ethanol were eluted at a flow rate of 1BV / h, and the eluted part of 60% ethanol was collected and evaporated to dr...

Embodiment 3

[0079] Extraction: Take 500g of Auranthus nudiflora leaves, add 7500mL of distilled water, soak for 2 hours, heat and extract for 2 hours, filter, add 6000mL of distilled water to the filter residue, heat and extract for 1 hour, combine 2 extracts, and filter the extract through 300 mesh gauze.

[0080] Impurity removal: heat and concentrate the filtrate to 500mL (1.0g / mL), add ZTC1+1 type II clarifier according to the volume ratio of liquid medicine and clarifier 1:0.12, stir for 10min at a temperature of 80°C, and stir at a speed of 100r / min. After standing still for 4 hours, centrifuge (3000r / min, 10min).

[0081] Refining: take the supernatant after centrifugation, add D-101 macroporous resin according to the amount of resin and medicinal material 1:1.2, and perform dynamic adsorption according to the adsorption flow rate of 1BV / h, and the ratio of diameter to height is 1:12, and use 4BV distilled water respectively , 5BV of 30% ethanol, and 8BV of 70% ethanol were eluted ...

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Abstract

The invention relates to the field of natural drug and specifically relates to a callicarpa nudiflora extract containing phenylethanoid glycoside and a preparation method thereof. The preparation method comprises the following steps of: heating callicarpa nudiflora leaves with water, carrying out reflux extraction, filtering the extracting solution, concentrating, adding a clarifying agent for clarifying the concentrated solution, enabling the clarified solution to pass through a macroporous resin column, firstly eluting with pure water, then, eluting with alcohol with low-concentration alcohol, finally, eluting with high-concentration alcohol, collecting the high-concentration alcohol eluant and drying, thus obtaining the callicarpa nudiflora extract. In the callicarpa nudiflora extract provided by the invention, total phenylethanoid glycoside content is up to 60-80%, and the sum of contents of three constituents including forsythiaside B, verbascoside and isoacteoside is up to 50%-70%.

Description

technical field [0001] The invention relates to the field of natural medicines, in particular to a phenylethanol glycoside-containing Auranthus nudiflora extract and a preparation method thereof. Background technique [0002] According to the statistics of the World Health Organization, there are nearly 350 million hepatitis B virus (HBV) carriers in the world, and there are 120 million in China. Infectious hepatitis B (viral hepatitis) has ranked the second in the incidence of infectious diseases in my country and the third in the number of deaths. Epidemiological surveys show that liver injury is one of the common clinical diseases that seriously endanger human health. At present, there are still great problems in the treatment of viral hepatitis. Although there are many drugs for clinical treatment of viral hepatitis B, there is no definite and effective drug. Comprehensive treatment is often advocated now. Although it can reduce liver damage, slow down the decline of l...

Claims

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Application Information

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IPC IPC(8): A61K36/85A61P1/16A61P31/20A61K127/00
Inventor 王勇陈峰谭银丰李永辉张小坡张俊清李友宾
Owner HAINAN MEDICAL COLLEGE
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