Plasma miRNA biomarker related to colorectal cancer and application thereof

Abstract

The invention provides a plasma miRNA biomarker related to colorectal cancer diagnosis and an application thereof, and belongs to the field of biotechnology and oncology. The plasma miRNA biomarker related to colorectal cancer diagnosis is plasma miR-21. The present study suggests that plasma miRNAs are mainly from secretion of tumor tissues or are induced by death and dissociation of tumor cells, but the miRNAs with differential expression in the tissues do not absolutely experience differential expression in the plasma, and some miRNAs are even reverse. Although the miR-21 has been determined to be capable of being used as the marker in colorectal cancer tissues, results in the reports of peripheral plasma samples are not uniform, the innovation of the invention lies in that the expression trend uniformity of the plasma miR-21 and the tissue miR-21 is determined, the plasma miR-21 can be used as a diagnostic marker and is non-invasive, so that the plasma miR-21 can be applied to future clinic.

Description

technical field [0001] The invention belongs to the fields of biotechnology and oncology, and relates to a plasma miRNA biomarker related to colorectal cancer and application thereof. Background technique [0002] Colorectal cancer (CRC) is one of the most common malignant tumors in the world, and its incidence rate ranks third among all tumors in the world. In Europe, America and other countries, the morbidity and mortality of colorectal cancer in all tumors account for about 8.6% and 8.8% respectively. From a global perspective, although China is an area with a low incidence of colorectal cancer, the incidence of colorectal cancer is increasing year by year. Early colorectal cancer lacks specific symptoms, and when patients seek treatment due to obvious clinical symptoms, they are often in the middle and advanced stages. About 25% of patients have metastases when they are first diagnosed, which seriously affects the treatment effect and prognosis. The high incidence of c...

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Problems solved by technology

[0003] Colonoscopy, double-contrast barium enema, and CT imaging are generally used in the diagnosis of colorectal cancer. However, these methods have certain defects in the early diagnosis of colorectal cancer in large populations: improper endoscopic detection or insufficient human experience may cause serious problems. Lead to misdiagnosis or missed diagnosis, and the discomfort of the endoscopic detection process further limits its wide application; imaging examination has certain limitations in the diagnosis of early colorectal cancer

[0006] Summarizing the existing research reports, although multiple circulating miRNAs are found to be suitable for the early diagnosis of colorectal cancer, there is controversy whether they are consistent with the expression trend in tissues, and the diagnostic value is single, different miRNAs lack diagnostic consistency, or the same miRNAs There are diagnostic differences, so it is necessary to comprehensively evaluate the application of circulating miRNAs for the early diagnosis of colorectal cancer

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