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RT-PCR primers for detecting transcriptional level of NOX1 mRNA in serum as well as kit and method for evaluating concurrent intestinal cancer susceptibility of hyperglycemia population

A RT-PCR, transcription level technology, applied in DNA/RNA fragments, biochemical equipment and methods, recombinant DNA technology, etc., can solve the problems of inaccurate mRNA quantification, easy contamination, false positive rate, application limitations, etc.

Inactive Publication Date: 2015-05-20
HUZHOU NO 1 PEOPLES HOSPITAL
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Problems solved by technology

However, in the application of traditional PCR technology, there are shortcomings such as inability to accurately quantify specific mRNA, and easy contamination during operation, resulting in a high false positive rate, so it cannot meet the needs of actual work and its application is limited.

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  • RT-PCR primers for detecting transcriptional level of NOX1 mRNA in serum as well as kit and method for evaluating concurrent intestinal cancer susceptibility of hyperglycemia population
  • RT-PCR primers for detecting transcriptional level of NOX1 mRNA in serum as well as kit and method for evaluating concurrent intestinal cancer susceptibility of hyperglycemia population
  • RT-PCR primers for detecting transcriptional level of NOX1 mRNA in serum as well as kit and method for evaluating concurrent intestinal cancer susceptibility of hyperglycemia population

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Embodiment Construction

[0074] In the following, the present invention will be further described in conjunction with embodiments. However, it should be clear that the above description and the following examples herein are only used as examples and do not constitute a limitation on the scope of the present invention.

[0075] The experimental methods used in the following examples are conventional methods unless otherwise specified.

[0076] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0077] 1. Primer design and synthesis

[0078] 1.1 Design and synthesis of primers for NOX1 gene

[0079] The standard sequence of NOX1 gene to be amplified is 266-422 bases in the second and third exon regions of NOX1 gene, and primers are designed using the full-length mRNA sequence of NOX1 as a template. The sequence of the upstream primer F of the standard PCR is: 5'-ATAGCAGAAGCCGACAGG -3'; the sequence of the downstream primer R is: 5'-CC...

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Abstract

The invention relates to an RT-PCR kit and a method for evaluating the concurrent intestinal cancer susceptibility of a hyperglycemia population by using the kit, belonging to the technical field of real-time fluorescence quantitative reverse transcription polymerase chain reaction kit development and human pathology combination. RT-PCR primers for detecting the transcriptional level of NOX1 mRNA in serum include a primer F: 5'-ATAGCAGAAGCCGACAGG-3' and a primer R: 5'-CCAGTGAGACCAGCAATG-3'. The invention establishes a method for detecting NOX1 mRNA in peripheral vein serum by using the specific primers; and a quantitative PCR amplification technology is adopted in the method, so that the sensitivity for detecting NOX1 mRNA is greatly improved, and enough information can be obtained in extremely few specimens.

Description

technical field [0001] The present invention relates to a primer, a kit and a method for assessing the susceptibility of hyperglycemia crowd complicated with colorectal cancer; in particular, it relates to a RT-PCR primer for detecting NOX1 mRNA transcription level in serum, which is used for assessing hyperglycemia crowd complicated with colorectal cancer RT-PCR kit for susceptibility and method for assessing susceptibility to colorectal cancer in hyperglycemia population. It belongs to the technical field of the combination of real-time fluorescent quantitative reverse transcription polymerase chain reaction kit development and human pathology. Background technique [0002] According to the statistics of the World Health Organization (WHO), more than 220 million people in the world suffer from diabetes, and 3.4 million people died of severe complications caused by high blood sugar in 2004. By 2030, the number of deaths due to diabetes will double again . Among the Chines...

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q1/6851C12Q2600/118C12Q2600/158
Inventor 崔戈鲍鹰冯文明郭慧慧薛涛王翔
Owner HUZHOU NO 1 PEOPLES HOSPITAL
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