CO2 fractional laser sterilization experimental method

Abstract

The invention discloses a CO2 fractional laser sterilization experimental method. The method includes: conducting aseptic operation on a first strain and a second strain, and inoculating the strains into a solid medium to conduct culture; extracting single bacterial colony of each strain from the solid medium, performing inoculation into 5ml liquid media respectively, and placing the media into a 37DEG C constant temperature shaking table to conduct culture; taking 2.5ml of a culture liquid and adding it into a 10ml liquid medium, and performing culture in a 37DEG C constant temperature shaking table; by means of turbid comparison with a Mcfarland turbidimetric tube, preparing the bacterial liquids of the liquid media into bacterial liquids of three concentrations; coating culture dishes with each concentration bacterial liquid of every strain; irradiating the centers of the bacteria coated culture dishes by CO2 fractional laser, employing CO2 fractional laser with the irradiation energy intensity of 3J, 8J and 12J respectively to conduct irradiation for 1ms respectively on the culture dishes corresponding to three concentrations of each strain; placing the irradiated culture dish for each concentration bacterial liquid of every strain into a 37DEG C bacteria incubator to conduct culture; and after irradiation, observing whether bacterial colonies grow on the laser irradiated culture dishes continuously, if any, counting bacteria and representing the number by CFU (colony-forming units).

Description

technical field [0001] The present invention relates to the field of medicine, in particular to a CO 2 Experimental method of fractional laser sterilization. Background technique [0002] CO 2 Laser was first invented by Kumar Patel in 1963 and developed rapidly in the 1970s. It was mainly used for skin damage such as skin warts and skin appendage tumors. By the 1980s, CO 2 Lasers are used to treat photoaging. After the 1990s, ultrashort pulse CO 2 and CW CO 2 The development of laser has greatly improved the effectiveness and safety of the laser, through rapid irradiation to reduce the area of ​​tissue vaporization and coking, reduce thermal damage, and reduce complications. [0003] With the development of fractional laser technology in 2003, CO 2 The fractional laser is introduced. CO 2 Fractional laser is an exfoliative fractional laser, which produces a micro-thermal area of ​​epidermis vaporization and exfoliation on the irradiated skin surface. The skin betwee...

AI Technical Summary

Problems solved by technology

Currently CO 2 Laser is mainly used for wrinkle removal, mole removal, acne scars, etc., but using CO 2 Laser sterilization is rarely reported

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