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Bacillus amyloliquefaciens RL263 capable of preventing and controlling rice blast

A technology of amylolytic spores and RL263, applied in the field of microorganisms, can solve the problems of control effects in the field and less research on actual production applications, and achieve the effect of prolonging the effective period of control and good colonization survival

Active Publication Date: 2015-05-20
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among the reported studies on the control of rice blast by Bacillus amyloliquefaciens, there are many studies on the antibacterial effect in the laboratory, but there are few studies on the field control effect and actual production application.

Method used

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  • Bacillus amyloliquefaciens RL263 capable of preventing and controlling rice blast
  • Bacillus amyloliquefaciens RL263 capable of preventing and controlling rice blast
  • Bacillus amyloliquefaciens RL263 capable of preventing and controlling rice blast

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Experimental program
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Effect test

Embodiment 1

[0029] Embodiment 1: Screening of Bacillus amyloliquefaciens RL263 bacterial strain

[0030] (1) Rice material: the sample used is rice leaves, the variety is Liaoxing 1, and the collection location is Donggang City, Liaoning Province.

[0031] (2) Medium used:

[0032] 1) Beef extract medium (NA): 3g beef extract, 7g soybean peptone, 5g NaCl, 20g agar, 1L dH 2 O, the pH value is 7.2.

[0033] 2) Beef extract liquid medium (NB): 3g beef extract, 7g soybean peptone, 5g NaCl, 1L dH 2 O, the pH value is 7.2.

[0034] 3) Tryptone liquid medium (LB): tryptone 10g, yeast powder 5g, NaCl 10g, 1L dH 2 O, the pH value is 7.2.

[0035] 4) Tomato oat culture medium: oat 40g, tomato juice 150mL, agar 20g, CaCO 3 0.6g, 1L dH 2 O.

[0036] (3) Isolation of Bacillus: (a) Rinse the rice leaves with clean water, disinfect with 1% sodium hypochlorite for 8 minutes, rinse with sterile water for 4 times, take 100 μl of the 4th rinse water and apply beef extract medium plate culture for 3 ...

Embodiment 2

[0044] Embodiment 2: the biological activity assay of Bacillus amyloliquefaciens RL263 bacterial strain:

[0045] (1) Control effect test of Bacillus amyloliquefaciens RL263 on rice leaf blast greenhouse potted plants: Lijiang Xintuan Heigu and Liaoxing No. 1 rice varieties were used as experimental materials, and the leaves were sprayed with 1% ammonium sulfate at the stage of four leaves and one heart. solution, after 3 days, select plants with consistent growth for biocontrol test. Prepare 1×10 5 The spore suspension of Magnaporthe oryzae of cfu·mL-1 was sprayed and inoculated on the RL263 strain, and kept in darkness for 12 hours after inoculation, and the humidity was 90%. After 2 hours, the rice plants inoculated with the pathogenic bacteria were sprayed with the fermentation liquid of the RL263 strain at a concentration of 1.0×10 8 cfu mL-1, 30 plants per treatment, the treatment of clear water spray as control 1, and the treatment of only inoculating Magnaporthe gris...

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PUM

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Abstract

The invention discloses bacillus amyloliquefaciens RL263 capable of preventing and controlling rice blast, as well as an inoculant containing the bacillus amyloliquefaciens RL263, a method for preventing and controlling rice blast by using the bacillus amyloliquefaciens RL263 and application of the bacillus amyloliquefaciens RL263 in prevention and control of rice blast. The bacillus amyloliquefaciens has an effective prevention and control effect on rice blast.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a Bacillus amyloliquefaciens for controlling rice blast and application thereof. Background technique [0002] Rice is one of the most important food crops in the world. In my country, the planting area of ​​rice is about 1 / 4 of the country's arable land, but the annual output accounts for half of the country's total grain output. In the rice production process, disease problems (especially rice blast) are one of the important obstacles restricting high and stable rice yields (Dong Jingao et al., 2008). [0003] Rice blast is a fungal disease caused by Magnaprthe grisea (Hebert) Barr (Sun Taihong, 2009). Rice blast is one of the most widespread and serious plant fungal diseases. According to the time and location of the disease, it can be divided into seedling blast, leaf blast, node blast, panicle blast, branch blast, grain blast, etc. Among them, leaf blast and panicle blast are the m...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00A01G7/06C12R1/07
CPCA01N63/00A01N63/10C12N1/20C12N1/205C12R2001/07
Inventor 王琦李双东李燕
Owner CHINA AGRI UNIV
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