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Anti-oxidative seaweed polypeptide and preparation method thereof

A technology of antioxidant polypeptides and seaweed, applied in the biological field, can solve the problems of low utilization rate of resources, waste of leftovers, environmental pollution, etc., and achieve the effects of improving the low utilization rate, eliminating side effects, and alleviating concerns about food safety.

Active Publication Date: 2015-05-20
FUZHOU UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] my country is rich in seaweed resources, but due to the low utilization rate of resources, the waste of leftovers generated in the processing process and other reasons cause waste of resources and even environmental pollution

Method used

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  • Anti-oxidative seaweed polypeptide and preparation method thereof
  • Anti-oxidative seaweed polypeptide and preparation method thereof
  • Anti-oxidative seaweed polypeptide and preparation method thereof

Examples

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Embodiment 1

[0047] Weigh 7.5 grams of laver protein, dissolve it in deionized water and make it up to 250ml, then adjust its pH to 4.0 with 2mol / L HCl. First heat the solution to 45°C in a water bath, then add a corresponding amount of enzyme according to the enzyme-substrate ratio of 3000U / g, and the enzymatic hydrolysis time is 7 hours. Then inactivate the enzyme in a boiling water bath for 15 minutes, then centrifuge at 4000 rpm for 15 minutes after cooling. Collect the supernatant for later use.

[0048] The supernatant was separated by ultrafiltration with an ultrafiltration membrane with a molecular weight cut-off range of 8000Da and 3500Da, and it was divided into three components with different molecular weight ranges, namely components with a molecular weight greater than 8000Da and components with a molecular weight between 3500Da and 8000Da. Components, components with a molecular weight less than 3500Da, and their antioxidant activity was determined. The components with mole...

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Abstract

The invention provides anti-oxidative seaweed polypeptide and a preparation method thereof. According to the method, by enzymolysis of acid proteinase, seaweed protein is taken as a raw material to be separated and purified to obtain specific anti-oxidative polypeptide of which the complete sequence of amino acids is avvkeagdacf. The anti-oxidative seaweed polypeptide disclosed by the invention has the advantages of eliminating defects of natural antioxidants, also eliminating worries of publics on artificial synthesis of antioxidants, and laying the foundation for developing the anti-oxidative polypeptide based on a food source and exploring wide application of the anti-oxidative seaweed polypeptide in foods and medical science.

Description

technical field [0001] The invention provides a seaweed antioxidant polypeptide and a preparation method thereof, belonging to the field of biotechnology. Background technique [0002] Oxidation of biomolecules is a free radical-mediated process that can have many adverse effects on food and biological systems. In aerobic organs, free radicals related to many diseases such as arteriosclerosis and cancer will inevitably be produced along with the process of oxygen metabolism. In food, the oxidation of food nutrients will produce peroxides, which will not only affect the nutritional value of food, cause food quality to decline, and even cause diseases in the body of the ingestor. Therefore, finding safe antioxidants to inhibit the production of peroxides has always been a research hotspot in biochemical nutrition. Because chemically synthesized antioxidants such as BHT and TBHQ have better effects and cheaper prices than natural antioxidants, they have been widely used in th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C12P21/06C07K1/36C07K1/34C07K1/20C07K1/18C07K1/16
Inventor 汪少芸吴妙鸿黄琦敏蔡茜茜付才力赵立娜
Owner FUZHOU UNIV
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