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Application of diclofenac sodium to preparation of medicine for inhibiting tumor cell metastasis and spreading

A technology of diclofenac sodium and tumor cells, applied in the pharmaceutical field of diclofenac sodium, can solve the problems such as the pharmacological effects of diclofenac sodium have not been found, and achieve the effects of reducing the number of deaths, large blocking effect, and shortening the research and development cycle.

Inactive Publication Date: 2015-05-20
南京格耀生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Other pharmacological effects of diclofenac sodium have not been found in the existing open literature

Method used

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  • Application of diclofenac sodium to preparation of medicine for inhibiting tumor cell metastasis and spreading
  • Application of diclofenac sodium to preparation of medicine for inhibiting tumor cell metastasis and spreading
  • Application of diclofenac sodium to preparation of medicine for inhibiting tumor cell metastasis and spreading

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Application of diclofenac sodium in the preparation of a drug for inhibiting the metastasis and spread of colon cancer cells. The specific experimental plan is as follows:

[0030] 1. Experimental materials

[0031] Colon cancer cells (HT29) were used in the experimental stage of the present invention, and the drug used in the positive control was nocodazole (experimental concentration: 10uM). The negative control group received no treatment.

[0032] The experiment used diclofenac sodium compound C 14 h 10 Cl 2 NNaO 2 , CAS NO. is 15307-79-6, the concentrations are 80uM, 60uM, 50uM, 40uM, 10uM.

[0033] 2. Experimental method

[0034] For colon cancer cells cultured for 24 hours (cell density: 10 5 ) for drug stimulation for 2 hours:

[0035] (1) Centrifuge the colon cancer cells in a better state in the culture dish, and add an appropriate amount of culture medium to make a cell suspension;

[0036] (2) Get the cell suspension count, according to ...

Embodiment 2

[0044] Example 2: Transwell cell migration assay was used to determine the effect of diclofenac sodium on the migration ability of colon cancer cells.

[0045] Will 1×10 5 The HT29 cells were inoculated in Transwell chambers with a 12.0 μm membrane at the bottom, and continued to be cultured. After the cells adhered to the wall, DMEM complete medium with different concentrations of diclofenac sodium was added to the upper chamber. To maintain osmotic pressure, 0.05%-0.2% BSA was added, and DMEM complete medium containing 15% FBS was added to the lower chamber to continue culturing for 24 hours. The unmigrated cells on the upper surface of the membrane were carefully wiped off with a cotton swab, and the cells that migrated to the lower surface of the membrane were fixed with 2% PFA at 37°C for 10 minutes and then stained with Giemsa. Ten visual fields were randomly selected under a high-power microscope to count the migrated cells.

[0046] Cell migration rate = [the average...

Embodiment 3

[0050] Example 3: The damage repair experiment detects the effect of diclofenac sodium on the migration ability of tumor cells.

[0051] Take HT29 cells in the logarithmic growth phase, and add 1×10 cells to each well of a 24-well plate5 After the cells grow into a single layer, draw a "one"-shaped scratch, rinse with PBS, add different concentrations of diclofenac sodium to the control group and the experimental group, and take pictures. After 22 hours of culture, take pictures again at the same site to measure the migration distance. The experiment was repeated 3 times, and the cell migration rate=[(0h average value of the experimental group-22h average value of the experimental group) / (0h average value of the blank control group-22h average value of the blank control group)]×100%.

[0052] Diclofenac sodium acts on LTEP and A549 cells for 22 hours at different concentrations (10-80 μg / ml) to inhibit migration. The results show that diclofenac sodium has a dose-dependent inhi...

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Abstract

The invention discloses application of diclofenac sodium to preparation of a medicine for inhibiting tumor cell metastasis and spreading. According the invention, the medicine prepared from diclofenac sodium is utilized to inhibit the metastasis capability of tumor cells; diclofenac sodium is a low-toxicity medicine and can play a great blocking role in the tumor spreading process, and meanwhile other related tumor medicines are adopted for treatment so that the mortality quantity of normal cells can be greatly reduced. Most conventional tumor medicines are cell poison and kill a great quantity of normal cells while killing tumor cells.

Description

technical field [0001] The invention relates to a new application of diclofenac sodium in the field of pharmacy, in particular to the application of diclofenac sodium in the preparation of drugs for inhibiting tumor cell metastasis and diffusion. Background technique [0002] Diclofenac sodium exerts antipyretic, analgesic and anti-inflammatory effects by inhibiting cyclooxygenase to reduce the synthesis of prostaglandins, and inhibiting lipoxygenase to a certain extent to reduce the production of leukotrienes, bradykinin and other products. Mainly used: [0003] 1. Inflammatory and degenerative rheumatic diseases: rheumatoid arthritis, juvenile rheumatoid arthritis, ankylosing spondylitis, osteoarthritis and spondyloarthritis. Spine Pain Syndrome. [0004] 2. Non-articular rheumatic diseases, such as shoulder pain, tenosynovitis, bursitis, myalgia and post-exercise injury pain, etc. [0005] 3. Acute attack of gout. [0006] 4. Post-traumatic and postoperative inflammat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/196A61P35/04
Inventor 董梅
Owner 南京格耀生物科技有限公司
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