Method for reducing content of protein in sodium hyaluronate prepared by bio-extraction method and prepared sodium hyaluronate

Abstract

The invention relates to a method for reducing the content of protein in sodium hyaluronate prepared by a bio-extraction method. At present, a comb extract method for producing the sodium hyaluronate usually comprises the following steps: coarse cleaning, fine cleaning and grinding, enzymolysis, primary adsorption, CPC precipitation and dissociation, secondary adsorption, alcohol precipitation, and drying to obtain sodium hyaluronate crude drug. According to the method, after performing the CPC precipitation and dissociation, purified water is added to a dissociation solution, so that the ion strength of the dissociation solution is reduced; based on the quaternary ammonium salt complex-precipitation dissociation principle, positive ions on a quaternary ammonium group is free under the low salt concentration and is combined with negative ions on a mucopolysaccharide molecule to form a quaternary ammonium complex, and the quaternary ammonium complex is gradually dissociated under the high salt concentration. By virtue of the reversible reaction, precipitates in the dissociation solution are repeatedly complexed, and then are subjected to the steps of rinsing, secondary dissociation, filtering, alcohol precipitation and drying so as to obtain the sodium hyaluronate crude drug. According to the method, the content of protein in the sodium hyaluronate can be remarkably reduced.

Description

Technical field: [0001] The invention relates to a method for reducing protein content in sodium hyaluronate prepared by biological extraction. Background technique: [0002] Sodium hyaluronate is a linear polysaccharide composed of (1→3)-2-acetylamino-2-deoxy-β-D-glucose-(1→4)-O-β-D glucuronic acid disaccharide repeating unit. glycans. The structure of HA is very regular, the same disaccharide unit is the basic structural unit of HA, and there is no species difference in HA from different animal tissues and bacteria. Non-antigenic to humans and animals. [0003] The chain length and amount of HA are not uniform. The amount ranges from 2×105 to 7×106, and the length of disaccharide units is 300-11000 pairs, which belongs to biological macromolecules. Commercial HA is generally in the form of sodium salt, a white fibrous or powdery solid, with strong hygroscopicity, soluble in water, insoluble in organic reagents, the medical fraction is 1×106~2.5×106, and the content is 9...

AI Technical Summary

Problems solved by technology

[0005] At present, the protein content of sodium hyaluronate produced by the traditional process is about 0.40-0.80%, which is relatively high, and cannot meet the new quality standard of sodium hyaluronate (the protein content does not exceed 0.1%)

Although the traditional secondary alcohol precipitation can significantly improve the protein content, it increases the production cost