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Plant-derived attractants of three species of Lygus genus Medicago

A technology of alfalfa mirid bugs and attractants, which is applied in the direction of attracting pests, plant growth regulators, animal repellants, etc., can solve the problems of increased harm, sharp increase in the population of mirid bugs, and reduced use of chemical agents

Active Publication Date: 2018-04-13
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, the large-scale planting of Bt cotton, which occurred in our country with less harm, greatly reduced the populations of major lepidopteran pests such as cotton bollworm, which led to a huge change in the use pattern of insecticides in Bt cotton fields, and the use of chemical agents Compared with before the commercial planting of Bt cotton, the use of Bt cotton has been significantly reduced. The population of mirid bugs, which were originally only secondary pests, has increased sharply, and the damage has increased. crops, has become a major issue affecting the production of a variety of crops

Method used

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  • Plant-derived attractants of three species of Lygus genus Medicago
  • Plant-derived attractants of three species of Lygus genus Medicago
  • Plant-derived attractants of three species of Lygus genus Medicago

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Collection of field host plant volatiles

[0021] When the main host plants of the three kinds of plant bugs such as field cotton are blooming, use a polyethylene fresh-keeping bag (40cm×60cm) to collect the volatiles at the flowering part, cut an oblique triangle at the upper corner of the fresh-keeping bag, and insert a piece of vented silica gel tube( 3350silicone, USA), wrapped with scotch tape to seal, insert an air inlet tube into the lower opening, and fix it on the plant with tape. The inlet and outlet silicone tubes are respectively connected to the outlet and the air sampler (Beijing Labor Protection Institute) Inlet, the air in the inlet silica gel tube is filtered by activated carbon and then enters the fresh-keeping bag. The air with plant volatile gas is absorbed by the 40mg adsorbent in the adsorption column ( Type Q 80-100Mesh, Bulk Packing Material, Altech Assoc, USA) is absorbed and flows back into the atmosphere sampler. The gas flow rate is...

Embodiment 2

[0022] Example 2 Screening and identification of active volatile components

[0023] Use a finger tube to take out a 6-10 day-old blind bug, distinguish the male and female, and then place it on ice. Put it under the dissecting microscope with the abdomen face up, press the abdomen with the insect needle, use a double-sided blade to quickly cut down the middle of the forefoot leg of each kind of lygus, and observe whether there is any tissue membrane on the part of the head. If the tissue fluid flows out, the membrane is intact and can be tested further; if the tissue membrane is ruptured, it cannot be used and needs to be prepared again. Then cut off a small part of the tips of the two antennae, and then use the prepared reference electrode (the capillary is immersed in 0.9% NaCl solution) to align the tissue membrane at the incision and gently insert it. Insert too deep, stop when you feel a slight resistance, connect the reference electrode with the head to the EAG micro-mani...

Embodiment 3

[0027] Example 3 Olfactory Behavioral Response

[0028] Before the test, the female and male adults of the monocephalus were starved for 4 hours (only a cotton ball dipped in pure water was provided during this period), and then used for the behavior determination of the Y-type olfactory instrument. A 5ml centrifuge tube (with the bottom cut off) connected to a tube arm of the Y-type olfactory instrument contains a slow-release material with 20μl of active substance standard (concentration of 100mg / ml) added; the centrifuge tube connected to the other tube arm contains dripped 20μl control paraffin oil sustained-release material. The air flow rate is set to 0.4L / min. Release a female or male worm into the Y-shaped tube base arm for observation, and start timing when the worm is at 2 / 3 of the base arm. The judgment is made according to the following criteria: within 3 minutes, when the bug crosses 3 cm of any tube arm and stays in this area for more than 5 seconds, it is recorde...

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PUM

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Abstract

The invention relates to plant-source attractants for three species of mirid bugs belonging to the genus Medicago sativa, which are: p-xylene, dibutyl ether, butyl acrylate, butyl propionate and butyl butyrate. Among them, butyl acrylate, butyl propionate or butyl butyrate all have significant attracting effect on adults of three kinds of mirid bugs, and can be used as a potential general plant-source attractant for three kinds of mirid bugs of the genus Medicago californica.

Description

Technical field [0001] The invention relates to an insect attractant, in particular to a plant-derived attractant for three kinds of Lygus Medicago sativa and its application. Background technique [0002] Adelphocoris suturalis (Jakovlev), Adelphocorislineolatus (Goeze) and Adelphocoris fasciaticollis (Reuter) belong to the genus Adelphocoris, with a wide range of host plants, 270 species, 245 species and 127 species respectively . In the past, the large-scale planting of Bt cotton has greatly reduced the population of major lepidopteran pests such as cotton bollworm. This has led to a huge change in the use of pesticides in Bt cotton fields. Compared with the commercial planting of Bt cotton, the amount of use has been significantly reduced. The lygus population, which was originally a minor pest, has increased sharply and its damage has increased. It has gradually become the major pest in cotton fields and spread to other agriculture such as dates, grapes, cherries, tea trees...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N37/06A01P19/00A01N37/02A01N31/14A01N27/00
Inventor 修春丽潘洪生陆宴辉林克剑杨益众
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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