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Method for discriminating cordyceps sinensis storage time

A technology of Cordyceps sinensis and storage time, which is applied in the field of identification of Cordyceps sinensis storage time, and can solve the problems of poor accuracy and dependence on experience, etc.

Active Publication Date: 2015-05-06
YICHANG SHANCHENGSHUIDU CORDYCEPS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the methods to identify the storage time of Cordyceps sinensis on the market are mainly empirical identification, which is distinguished by observing its appearance and color. These methods rely on experience, and the accuracy is not easy to grasp

Method used

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  • Method for discriminating cordyceps sinensis storage time
  • Method for discriminating cordyceps sinensis storage time
  • Method for discriminating cordyceps sinensis storage time

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Thin-layer chromatography (TLC) identification of different Cordyceps sinensis and observation of samples damaged by ergosterol at high temperature

[0034] Take Cordyceps sinensis at room temperature for 2 months, and Cordyceps sinensis for 22 months at room temperature. After crushing, take about 0.1g each, add 3ml of methanol, ultrasonically extract for 30min, filter and concentrate to 0.1ml, and centrifuge to take the supernatant as the test solution; Put about 50 mg of the ergosterol reference substance at 105°C and bake for 8 hours, add 1 ml of methanol, shake and shake well, filter, and take the subsequent filtrate as the ergosterol high-temperature destruction sample solution. Simultaneously prepare ergosterol reference substance solution, blank solution, and spot III solution, developer is petroleum ether: ethyl formate: formic acid = 15:5:1, bake at 105°C for 15min, and detect the chromatogram under 365nm ultraviolet lamp Such as figure 1 .

[0035] From fi...

Embodiment 2

[0037] Identifying Cordyceps with Known Shelf Dates

[0038] Take Cordyceps sinensis for 1 month at room temperature, Cordyceps sinensis for 7 months at room temperature, Cordyceps sinensis for 12 months at room temperature, Cordyceps sinensis for 22 months at room temperature, Cordyceps sinensis for 27 months at room temperature, Qinghai Cordyceps sinensis (current year), take about 0.1g each after crushing , add methanol 3ml, after ultrasonic extraction for 30min, after high-speed centrifugation, take the supernatant and evaporate to dryness, add methanol 0.1ml to dissolve the residue, centrifuge and take the supernatant as the test solution, and prepare blank solution, ergosterol reference substance solution, and Solution for Spot III. The developer is petroleum ether: ethyl formate: formic acid = 15:5:1, baked at 80°C for 30 minutes, and detected under a 365nm ultraviolet lamp, the chromatogram is as follows figure 2 .

[0039] From figure 2It can be seen that with th...

Embodiment 3

[0041] Identification of Cordyceps sinensis with unknown storage date

[0042] Take commercial Cordyceps sinensis samples, Qinghai wild samples, Tibet Nagqu samples, Sichuan Ganzi samples, and Cordyceps sinensis reference medicinal materials respectively, take about 0.1g each after crushing, add acetone 3ml, ultrasonically extract for 30min, and after high-speed centrifugation, take the supernatant and evaporate to dryness. Add 0.1ml of acetone to dissolve, centrifuge for 5min (10000rpm), take the supernatant as the test solution, prepare blank solution, ergosterol reference solution, and spot III solution at the same time, and spot the plate at the same time. The developer is n-hexane: formic acid Ethyl ester: formic acid = 15:5:1, baked at 105°C for 15 minutes, and detected under 365nm ultraviolet lamp, the chromatogram is as follows image 3 .

[0043] From image 3 It can be seen that the commercially available Cordyceps is the newest, followed by Nagqu in Tibet, wild in...

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Abstract

The invention discloses a method for discriminating cordyceps sinensis storage time. The method comprises the following steps of extracting cordyceps sinensis powder by an organic solution, carrying out filtration or centrifugation, then carrying out condensation, carrying out thin layer chromatography on the solution and an ergosterol contrast, taking out a thin layer plate, carrying out heating and carrying out inspection and determination under the action of a ultraviolet light lamp. Through positions and brightness comparison of spots of a sample on the thin layer plate, substantial acquisition year of cordyceps sinensis can be known. The method has simple experiment processes, is free of an expensive detection apparatus and has a practical value in cordyceps sinensis market transaction.

Description

technical field [0001] The invention relates to the field of analysis of Chinese herbal medicines, in particular to a method for identifying the storage time of Cordyceps sinensis. technical background [0002] Cordyceps sinensis is a complex of the subpodia and larval corpses of the ergot fungus Cordyceps sinensis parasitizing on the larvae of the bat moth family insects. Cordyceps sinensis is regarded as a precious Chinese herbal medicine because of its immune regulation, anti-fatigue, anti-tumor, anti-aging and other functions, and is famous all over the world. In recent years, although Cordyceps sinensis resources are scarce and expensive, it still attracts attention for its various medicinal effects. [0003] The storage points of Cordyceps are moisture-proof, moth-proof and insect-proof. The common storage method of Cordyceps is to put it in a cool and dry place in a sealed container, and you can also add a desiccant to the container. It has been reported in the lit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N30/95
Inventor 李文庆钱正明李文佳孙培培徐军
Owner YICHANG SHANCHENGSHUIDU CORDYCEPS
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