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Method for enhancing high-quality gelatin yield

A gelatin, high-quality technology, applied in the field of improving the yield of high-quality gelatin, can solve the problems of not being able to guarantee the complete extraction of gelatin, increasing the extraction process, and affecting the quality of the gelatin

Active Publication Date: 2015-05-06
HUNAN ER KANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the specificity of the enzyme, a single enzymatic extraction cannot guarantee the complete extraction of the gel, and the extraction process is undoubtedly increased when the process of extracting the gel is divided into different stages (Patent Publication No. CN 101107974)
The control of the end point of enzymatic hydrolysis is also a key step in enzymatic gel extraction. The common practice is to increase the temperature to 80-100°C instantaneously (patent publication number CN 101107974) or adjust the pH of the gel solution to 1-2 (patent publication No. CN 101637216A), these will affect the quality of glue

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The present embodiment adopts the pigskin of 1 kg, prepares gelatin by the method of the present invention, comprises the following steps;

[0023] (1) Enzymatic hydrolysis and gel extraction: adjust the pH of the gel solution to 4.0 with citric acid, add 10ppm cathepsin, the addition amount is 0.03% of the raw material weight, keep the temperature at 45°C, and the reaction time is 1 hour; add calcium hydroxide solution or Ammonia water, adjust the pH to 6.0, add 30ppm neutral protease, the addition amount is 0.01% of the raw material weight, keep the temperature at 50°C, and enzymolysis for 1.0 hour; add calcium hydroxide alkali solution or ammonia water to adjust the pH of the glue to 7.0, and the temperature rises to 50°C to inactivate the protease, add 50ppm trypsin to the reaction, keep the temperature at 50°C, and perform enzymatic hydrolysis for 2 hours.

[0024] (2) Removal of impurities: During post-treatment, chitosan, sodium alginate, divinylbenzene cross-lin...

Embodiment 2

[0029] The present embodiment adopts the cowhide of 1 kg, prepares gelatin by the method of the present invention, comprises the following steps;

[0030] (1) Enzymatic hydrolysis and gel extraction: adjust the pH of the glue solution to 5.0 with citric acid, add 10ppm papain, the addition amount is 0.04% of the raw material weight, keep the temperature at 50°C, and the reaction time is 1.5 hours; add calcium hydroxide solution or Ammonia water, adjust the pH to 7.0, add 30ppm neutral protease, the addition amount is 0.02% of the raw material weight, keep the temperature at 55°C, and enzymolysis for 1.5 hours; add calcium hydroxide alkali solution or ammonia water to adjust the pH of the glue to 7.5, and the temperature rises To 55°C, inactivate the protease, add 50ppm trypsin to the reaction, keep the temperature at 55°C, and enzymatically hydrolyze for 2.5 hours.

[0031] (2) Removal of impurities: During post-treatment, chitosan, sodium alginate, divinylbenzene cross-linked...

Embodiment 3

[0036] In this embodiment, 10 kg of bone bone is used to prepare gelatin through the method of the present invention, which includes the following steps;

[0037] (1) Enzymatic hydrolysis and gel extraction: adjust the pH of the gel solution to 6.5 with citric acid, add 10ppm papain, the addition amount is 0.05% of the raw material weight, keep the temperature at 55°C, and the reaction time is 2 hours; add calcium hydroxide solution or Ammonia, adjust the pH to 8.0, add 30ppm neutral protease, the addition amount is 0.03% of the raw material weight, keep the temperature at 60°C, enzymolysis for 2 hours; the temperature rises to 60°C to inactivate the protease, add calcium hydroxide alkali solution Or adjust the pH of the glue solution to 8.0 with ammonia water, add 50ppm trypsin to the reaction, keep the temperature at 60°C, and enzymatically hydrolyze for 3 hours.

[0038] (2) Removal of impurities: In the post-treatment process, add chitosan, sodium alginate, divinylbenzene ...

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Abstract

The invention discloses a method for enhancing high-quality gelatin yield, which comprises the following steps: regulating the pH value of a colloidal liquid obtained by a pretreatment process, adding biological proteinase by stages to perform colloidal extraction, adding a bamboo charcoal-chitosan composite into the obtained colloidal liquid to perform adsorption filtration, bleaching and sterilizing with hydrogen peroxide, carrying out impurity removal and concentrating by a membrane process, and finally, drying with a flat plate to obtain the gelatin finished product. The stage-by-stage enzyme addition and colloidal extraction are adopted to completely hydrolyze collagens, so that the gelatin yield is greatly enhanced; and the bamboo charcoal-chitosan composite adsorption filtration, H2O2 treatment, membrane-process impurity removal and concentration and other steps are performed to obtain the high-viscosity high-jelling-power gelatin product.

Description

technical field [0001] The invention relates to a method for increasing the yield of high-quality gelatin, and belongs to the technical field of gelatin making. . Background technique [0002] Gelatin is a polymer compound, and its main raw materials are animal skin, bone, cartilage, ligament, sarcolemma, etc. The traditional methods of extracting gelatin are acid method and alkali method. Since the 1950s, people have tried to introduce the protease-catalyzed degradation of collagen into the production of gelatin. Compared with the traditional process, it has the advantages of short cycle and easy equipment. Control and less environmental pollution and other advantages. [0003] At present, enzymatic gel extraction is divided into single enzymatic extraction and combined enzyme extraction. Due to the specificity of enzymes, a single enzymatic extraction cannot guarantee the complete extraction of gelatin, and when the process of extracting gelatin is divided into differen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 帅放文王向峰章家伟
Owner HUNAN ER KANG PHARMA
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