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Method for preparing spores of bacillus stearothermophilus

A technology of thermophilic steatospores and thermophilic fats, applied in the field of microbial spore induction, to achieve the effects of short production cycle, simple process conditions and high purity

Active Publication Date: 2015-05-06
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, in the prior art, it has been possible to prepare small doses of spores of Bacillus stearothermophilus, but in large-scale mass production, there are many technical obstacles: 1) how to easily and efficiently produce Scraping a large number of strains with high activity and high yield; 2) How to develop a low-cost, suitable for large-scale production of sporulation-inducing medium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for preparing the spores of bacillus stearothermophilus, comprising the following steps:

[0032] Step 1) Strain activation: use Geobacillus stearothermophilus as the strain, inoculate the strain on the surface of the activation medium, and cultivate it at 50° C. for 12 hours. At this time, the strain grows a lawn; the activation medium consists of the following: The material composition of parts by weight: 10 parts by weight of tryptone, 5 parts by weight of soybean peptone, 5 parts by weight of casein peptone, 5 parts by weight of sodium chloride, 15 parts by weight of agar and 1000 parts by weight of distilled water;

[0033] Step 2) Bacterial proliferation: Add the activated medium containing the lawn obtained in the previous step to the proliferation medium, and cultivate it for 12 hours at 50° C. under the condition of a rotating speed of 100 rpm / min; the proliferation medium consists of the following materials in parts by weight: Composition: 5 parts by ...

Embodiment 2

[0037] A method for preparing the spores of bacillus stearothermophilus, comprising the following steps:

[0038] Step 1) Strain activation: with Geobacillus stearothermophilus as the strain, the strain was inoculated on the surface of the activation medium, and after culturing at 60°C for 24 hours, the strain grew a lawn; the activation medium consisted of the following: The material composition of parts by weight: 10 parts by weight of tryptone, 5 parts by weight of soybean peptone, 5 parts by weight of casein peptone, 5 parts by weight of sodium chloride, 15 parts by weight of agar and 1000 parts by weight of distilled water;

[0039] Step 2) Bacterial proliferation: Add the activated medium containing the lawn obtained in the previous step to the proliferation medium, and cultivate it for 12 hours in an environment of 60° C. at a speed of 250 rpm / min; the proliferation medium consists of the following materials in parts by weight: Composition: 10 parts by weight of trypton...

Embodiment 3

[0043] A method for preparing the spores of bacillus stearothermophilus, comprising the following steps:

[0044] Step 1) Strain activation: use Geobacillus stearothermophilus as the strain, inoculate the strain on the surface of the activation medium, and cultivate it at 58° C. for 18 hours. At this time, the strain grows a lawn; the activation medium consists of the following: The material composition of parts by weight: 10 parts by weight of tryptone, 5 parts by weight of soybean peptone, 5 parts by weight of casein peptone, 5 parts by weight of sodium chloride, 15 parts by weight of agar and 1000 parts by weight of distilled water;

[0045] Step 2) Thalline proliferation: Add the activated medium containing the lawn obtained in the previous step into the proliferation medium, and cultivate it for 12 hours at 58° C. under the condition of a rotating speed of 200 rpm / min; the proliferation medium consists of the following materials in parts by weight: Composition: 7.5 parts ...

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Abstract

The invention discloses a method for preparing the spores of bacillus stearothermophilus. The method comprises the steps of culture activation, thallus multiplication, thallus screening and spore induction, wherein 1-5 pieces of sterile filter paper cover the surface of a culture medium during the thallus screening, and 5-10 parts of PEG6000 by weight is added in the culture medium during the spore induction. According to the method disclosed by the invention, the spores can be massively prepared in a short time by improving the formula of the culture medium and the culture conditions; moreover, the culture medium is prepared from cheap and easily available ingredients under simple process conditions within a short production period; the time consumption of one batch of the whole preparation process is within 90 hours; the scheme provides a simple culture method and an industrialized fermentation cultivation route, and the spores produced by induction are high in purity and has a content of 1*10<9> / mL and a conversion rate of greater than 98%.

Description

technical field [0001] The invention relates to the field of microbial spore induction, in particular to a method for preparing spores of Bacillus stearothermophilus, in particular to a method for preparing spores of Bacillus stearothermophilus which can be used in large-scale production. Background technique [0002] Bacillus stearothermophilus is a Gram-positive bacterium, aerobic or facultatively anaerobic, using glucose to produce acid but not gas. Bacillus stearothermophilus can produce spores, the optimum growth temperature is 55-60°C, and the highest growth temperature is 65-75°C, which belongs to thermophilic bacteria. The collection number of China Industrial Microbiology Culture Collection Center (CICC) is CICC: 10267, namely ATCC: 7953. [0003] The spores produced by Bacillus stearothermophilus are non-pathogenic, non-pyrogenic and non-toxic. Its spores have certain resistance to certain sterilization factors, especially to pressure steam, which is stronger tha...

Claims

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Application Information

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IPC IPC(8): C12N3/00C12R1/07
Inventor 尹焕才田晶晶
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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