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Application of Acetate Ceramide in Marine Fouling Biocontrol

A technology of acetic acid amide and marine fouling, which is applied in the separation/purification of carboxylic acid amide, paints containing biocide, anti-fouling/underwater coatings, etc., and can solve the problems such as the anti-adhesion effect of acetic acid amide that has not been reported. , to achieve good anti-bioadhesion activity, good application prospects, and simple preparation method

Active Publication Date: 2017-06-09
LINGNAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Amides are a class of compounds with a peptide bond structure and are widely distributed in seaweeds. They have biological activities such as antioxidant, anti-inflammatory, antibacterial and insecticidal effects. However, in the field of marine fouling biocontrol, no reports have disclosed peracetic acid Anti-adhesion effect of orange amide and its use in biofouling control

Method used

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  • Application of Acetate Ceramide in Marine Fouling Biocontrol
  • Application of Acetate Ceramide in Marine Fouling Biocontrol
  • Application of Acetate Ceramide in Marine Fouling Biocontrol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Extracting acetic acid orange amide from Naozhou Sargassum, comprising the following steps:

[0038] S1. Sargassum Naozhou was extracted three times with 95% alcohol to obtain an extract;

[0039] S2. After the extract is concentrated under reduced pressure, it is dispersed in water, and extracted three times with petroleum ether and ethyl acetate respectively;

[0040] S3. Get the sherwood oil extraction part through normal phase silica gel column chromatography, the eluent used during chromatography is the gradient eluent that normal hexane and acetone form, and wherein normal hexane: the volume ratio of acetone is respectively 10:0,8: 2. 4:6, 6:4, 2:8, 0:10, eluted to get 10 components F1~F10;

[0041] S4. Get the component F7 wherein and then purify through gel column chromatography. The eluent used during chromatography is a mixed solution of chloroform and methanol, wherein chloroform: the volume ratio of methanol is 1:1, and then purified by recrystallization, t...

Embodiment 2

[0044] Experimental group I: the orange amide acetate extracted in Example 1 was dissolved in chloroform to prepare a solution with a concentration of 28.26 μg / ml. Add 1 ml of this solution to a petri dish with a diameter of 6 cm, and make it evenly cover the bottom of the petri dish. After the solvent is completely evaporated, the content of acetic acid orange amide coated on the bottom of the culture dish is 1 μg / cm 2 . Add 10 ml seawater.

[0045] Experimental group II: the orange amide acetate extracted in Example 1 was dissolved in chloroform to prepare a solution with a concentration of 2.826 μg / ml. Add 1 ml of this solution to a petri dish with a diameter of 6 cm, and make it evenly cover the bottom of the petri dish. After the solvent is completely evaporated, the content of acetic acid orange amide coated on the bottom of the culture dish is 0.1 μg / cm 2 . Add 10 ml seawater.

[0046] In the present invention, the acetate orange amide forms a coating on the botto...

Embodiment 3

[0054] Experimental group: the orange amide acetate extracted in Example 1 was dissolved in chloroform to prepare a solution with a concentration of 28.26 μg / ml. Add 1 ml of this solution to a petri dish with a diameter of 6 cm and make it evenly cover the bottom of the petri dish. After the solvent is completely evaporated, the content of acetic acid orange amide coated on the bottom of the culture dish is 1.0 μg / cm 2 . Add 10 ml seawater.

[0055] In the present invention, the acetate orange amide forms a coating on the bottom of the culture dish, and the acetate orange amide is insoluble in seawater, and the purpose of adding seawater is to cultivate marine fouling organisms.

[0056] Control group: add 1 ml of chloroform to distribute the solution evenly on the bottom of the petri dish, and then add 10 ml of seawater after the solvent has evaporated completely.

[0057] Blank group: add 10 ml of sea water.

[0058] Determination of the number of larvae: Take the water ...

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Abstract

The invention belongs to the biotechnical field and particularly discloses an application of aurantiamide acetate in preventing and removing marine fouling organisms. According to the invention, aurantiamide acetate can effectively inhibit a setting and metamorphosing process of various larval marine fouling organisms and low dosage aurantiamide acetate has a remarkable inhibiting effect on attachment of marine organisms, and moreover, aurantiamide acetate inhibiting attachment of the marine fouling organisms does not pollute a water environment and is not enriched in living bodies due to transport through food chain. Therefore, aurantiamide acetate is a natural and environment-friendly compound which has good biofouling activity and has a good application prospect in preventing and removing marine fouling organisms for various marine facilities.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of acetic acid orange amide in biological control of marine fouling. Background technique [0002] Marine fouling organisms refer to all kinds of organisms that are fixed or inhabit the underwater parts of ships and marine facilities. Fouling organisms are usually divided into two life stages. The second is the fixation or attachment life stage, that is, the stage in which the larva chooses a place to settle, attaches to the surface of the attachment base, and metamorphoses to form a larvae. The hazards caused by marine fouling organisms mainly include: increased resistance, reduced speed, increased fuel consumption and CO 2 Discharge; block the seawater pipeline system, change the metal corrosion process, cause local corrosion or perforation corrosion; increase the dynamic load effect, cause drift, imbalance or even overturn of the facility; compete with...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09D5/16C07C237/22C07C231/24
CPCC07C231/24C09D5/1625C07C237/22
Inventor 彭燕曹文浩严涛童敏陈华絮胡小军
Owner LINGNAN NORMAL UNIV
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