Establishing method of primary generation humanized mammary cancer transplantation tumor model based on Chinese population and model

Abstract

The invention discloses an establishing method of a primary generation humanized mammary cancer transplantation tumor model based on Chinese population. The method comprises the following steps: (1) acquiring a human mammary cancer tissue sample; (2) performing inoculation for the first time; (3) culturing a mouse of the first inoculation; (4) performing inoculation for the second time; (5) culturing a mouse of the second inoculation; (6) performing inoculation for the third time; and (7) culturing a mouse of the third inoculation. The invention also discloses the primary generation humanized mammary cancer transplantation tumor model based on the Chinese population, which is obtained by the method. The primary generation humanized mammary cancer transplantation tumor model based on the Chinese population can be used for screening anti-mammary cancer medicaments and used as a test animal in a pharmacodynamics research. The primary generation humanized mammary cancer transplantation tumor model disclosed by the invention overcomes the problem that the existing transplantation tumor model in the Chinese population cannot truly reflect biological characteristics of the mammary cancer in a human body. Moreover, compared with the establishment of a foreign primary generation humanized mammary cancer related model, the primary generation humanized mammary cancer transplantation tumor model disclosed by the invention is used for realizing long-term and stable stimulation on the growth of primary generation humanized mammary cancer transplantation tumor and improving the method for establishing the model.

Description

technical field [0001] The invention relates to the technical field of medical evaluation and detection, in particular to a method and model for establishing a primary human breast cancer xenograft model based on a Chinese population. Background technique [0002] Among malignant tumors of the female reproductive system, breast cancer accounts for about 7% to 10% of all malignant tumors in the whole body. The incidence of breast cancer is on the rise all over the world. No matter in high-incidence areas or low-incidence areas, the incidence of breast cancer is increasing at a rate of 5-20%. [0003] Animal models of breast cancer are widely used in anti-breast cancer drug screening and pharmacodynamic research, and many literatures have reported the application of existing breast cancer animal models in anti-breast cancer drug screening and pharmacodynamic research. The production of breast cancer animal models plays a key role in the study of the etiology and pathogenesis ...

AI Technical Summary

Problems solved by technology

Although there have been many reports on the successful reconstruction of the human immune system and transplantation of human tumors using Nod-SCID mice, many mutations have been accumulated due to the excessive passage of the cell line, that is, its physiological characteristics have changed in many ways, many of which are in our real human beings. The biological characteristics of breast cancer in vivo cannot be studied with the cell line xenograft tumor model, and many research results obtained on the cell line xenograft tumor model cannot be used in clinical patient treatment practice

However, there are no reports at home and abroad on the primary human breast cancer xenograft model based on the Chinese population.

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