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Establishment method for experimental plateau intestinal barrier injury rat model

A technology of intestinal barrier and rat model, applied in the field of medicine, can solve the problems of restricting the mechanism of disease occurrence, the exploration of the development process and the evaluation of the treatment effect

Active Publication Date: 2015-04-29
中国人民解放军西部战区总医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, at present, there are few stable and reproducible experimental high-altitude gastrointestinal injury animal models at home and abroad that meet the actual physiological conditions of the human body after entering the plateau, which seriously restricts people's research on the mechanism, development process and therapeutic effect of this type of disease. Evaluation

Method used

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  • Establishment method for experimental plateau intestinal barrier injury rat model
  • Establishment method for experimental plateau intestinal barrier injury rat model
  • Establishment method for experimental plateau intestinal barrier injury rat model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Constructing an animal model

[0030] (1) Plain adaptive training stage

[0031] Before the exercise intervention, the two groups of animals were raised in the experimental site (Chengdu, May, altitude about 500m, temperature 24C°) for 1 week to eliminate the disturbance of the unfamiliar environment to the animals after the start of the experiment, no restriction on drinking water, and natural light. On the 8th day, all rats started adaptive training on the animal treadmill at 8:00 in the morning, in order to avoid the cooperation obstacle caused by the animals not familiar with the experimental equipment during the formal exercise intervention. The specific implementation method is: use the ZH-PT type animal experiment On the treadmill (10 tracks in total), adjust the conveyor belt slope to 0°, the speed at 8m / min, the time is 30min, and then rest for 30min, during which time you can eat and drink freely. Repeat the training 8 times in this way, the trainin...

Embodiment 2

[0036] Example 2 Detection of animal models

[0037] (1) Sample collection

[0038] After the experiment, group C collected materials in the plain environment, and group H quickly collected materials at a simulated altitude of 4000m. The weight of the rats was accurately weighed, and anesthetized by intraperitoneal injection of 10% ureose at 1 g / kg·BW. After the corneal reflex disappeared, the rats were fixed on the animal operating table in a supine position, and strictly aseptically operated. 20 rats were taken from each group, and animal blood, liver, spleen, mesenteric lymph nodes, and jejunum were collected in an ultra-clean workbench, and stored in liquid nitrogen for testing. The remaining 15 rats in each group were anesthetized in the same way, and a 2cm-long jejunum was cut at a distance of 10cm from the pylorus, cut longitudinally and trimmed into 1mm×1mm×1mm specimens, immersed in corresponding fixatives, and pathologically observed later.

[0039] (2) Bacterial t...

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Abstract

The invention belongs to the field of medicine technology, which relates to a medical animal model, in particular to an establishment method for a plateau intestinal barrier injury rat model. The method comprises the following steps of: firstly, feeding healthy rats for 5-7 days in a plain environment, then carrying out the animal treadmill adaptive training to obtain plain adaptive rats, and then carrying out plain intervention training for the plain adaptive rats by applying exercise load, so that the plain adaptive rats can reach to be in a fatigue stress state; continuously feeding the rats which reach to be in the fatigue stress state in a plateau hypobaric hypoxia simulation environment to obtain the experimental plateau intestinal barrier injury rat model. The method can establish the stable plateau intestinal barrier injury rat model, so that the experimental foundation for further research on the plateau intestinal injury mechanism is laid.

Description

technical field [0001] The invention belongs to the technical field of medicine and relates to a medical animal model, in particular to a method for establishing an experimental plateau intestinal barrier injury rat model. Background technique [0002] A large number of studies have already confirmed that when the body is subjected to major stress (such as trauma, surgery, shock, etc.), the intestinal tract is often one of the most sensitive organs. In addition to the damage caused by the stress itself, it is also easy to suffer from the expanded systemic inflammatory response. The "second hit" leads to the translocation of intestinal toxins and bacteria, and even induces multiple organ failure. The plateau is a special environment of hypobaric hypoxia, and there is no doubt that acute hypoxia is also a strong stimulus to the body, and acute hypoxia can cause obvious damage to various organs of the body. The special blood supply and anatomical structure of the intestinal tr...

Claims

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Application Information

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IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 罗涵肖贞良陈章李志伟周其全赵亮张竹青段玲祝国芸
Owner 中国人民解放军西部战区总医院
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