Detecting method for residual quantity of flubendiamide in fruits and vegetables

Abstract

The invention discloses a detecting method for the residual quantity of flubendiamide in fruits and vegetables. The detecting method comprises the steps of homogenizing by using acetonitrile or an acetonitrile liquid containing 1% acetic acid to extract residual flubendiamide in a sample; dispersing by using a primary secondary amine (PSA) and octadecylsilane bonded-phase (C18) matrix to purify an extracting liquid; then, carrying out gas chromatography-negative chemical ionization-mass spectrum (GC-NCI-MS) detection; establishing a corrected standard curve by using a blank matrix liquid without a pesticide to be detected; and quantifying by using an external standard method. By using the detecting method, the average recovery rate is 93.2-103.1%, the average relative standard deviation (RSD) is 4.6-7.4%, and the detection limit is lower than 1.15mu g / kg; and the detecting method is simple and convenient to operate, rapid, high in sensitivity, good in repeatability and accurate in qualitation and quantitation. According to the detecting method, the technical requirements of America, Japan, European union and other countries on security detection of corresponding products can be met, and the powerful technical support can be provided for ensuring Chinese food safety and the health development of foreign export trade.

Description

technical field [0001] The invention relates to a method for determining the residual amount of flubendiamide in vegetables and fruits, more specifically, it uses gas chromatography-negative chemical ion source-mass spectrometry (GC-NCI-MS) to qualitatively and quantitatively determine the residual amount of flubendiamide in vegetables and fruits. A method for flubendiamide content belongs to the technical field of pesticide residue determination. Background technique [0002] Flubendiamide (Flubendiamide), trade name Longge, is a new type of phthalamide insecticide developed by Japanese pesticide company (known as Co., Ltd.), which has good activity against almost all lepidopteran pests , not only has excellent activity on both adults and larvae, but also has a fast acting speed and a long lasting effect. The effect on rice stem borer and leaf roller is absolutely top-notch. The toxicity to bees is very low, and the toxicity to carp (a representative of aquatic organisms)...

AI Technical Summary

Problems solved by technology

Now various testing institutions and enterprises have purchased gas chromatography-mass spectrometers (GC-MS), and are generally equipped with negative chemical ion sources (NCI). Now many types of pesticides contain electronegative groups, organochlorines and pseudo Pyrethrin pesticide molecules mostly contain strong electronegative groups such as -F, -Cl, -Br or -COO-; organophosphorus pesticide molecules mostly contain =S, -OR, -P, -O-, -Cl, or -P=O and other electronegative groups; and most of the new pesticides developed in recent years contain -F groups. Therefore, the use of GC-NCI-MS can facilitate the multi-residue analysis of various pesticides. Compared with MS, it can obtain better anti-interference ability, lower sensitivity and better selectivity. Flubendiamide is an electronegative compound. GC-NCI-MS detection method is reported, therefore, it is of great significance to establish a gas chromatography-negative chemical ion source-mass spectrometry (GC-NCI-MS) detection method for the qualitative and quantitative analysis of flubendiamide residues in vegetables and fruits

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