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Tobacco nicotine synthesis related long non-coding RNA gene and application of tobacco nicotine synthesis related long non-coding RNA gene

A nicotine, non-coding technology, applied in the field of gene technology and botany, to achieve good tobacco nicotine content traits, improve the effect of tobacco nicotine content traits

Active Publication Date: 2015-04-08
ZHEJIANG UNIV
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  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Whether endogenous target mimics play a role in nicotine anabolism has not been reported

Method used

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  • Tobacco nicotine synthesis related long non-coding RNA gene and application of tobacco nicotine synthesis related long non-coding RNA gene
  • Tobacco nicotine synthesis related long non-coding RNA gene and application of tobacco nicotine synthesis related long non-coding RNA gene
  • Tobacco nicotine synthesis related long non-coding RNA gene and application of tobacco nicotine synthesis related long non-coding RNA gene

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Experimental program
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Embodiment Construction

[0018] The invention includes the non-coding eTM found in tobacco to regulate the function of tobacco nicotine metabolism miRNA, and provides a good basis for further research on the role played by the gene in the growth and development of tobacco. The research carried out in the present invention can help us to better understand the gene, and to further control the synthetic nicotine content of tobacco by means of molecular breeding (such as transgene and molecular marker-assisted breeding).

[0019] Materials and methods

[0020] 1. Identification of eTM by bioinformatics method

[0021] By using tobacco EST sequences from Genbank and identified miRNA sequences that regulate tobacco nicotine anabolism, a perl script was written for eTM prediction. The written perl script follows the following rules: (1) only the 9th to 12th positions on the miRNA 5' end sequence are allowed to bulge; (2) the bulge in the eTM consists of three nucleotides ; (3) The 2nd to 8th positions at ...

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Abstract

The invention relates to the field of genetic technologies and botany, and aims at providing a tobacco nicotine synthesis related long non-coding RNA gene and application of the tobacco nicotine synthesis related long non-coding RNA gene. The sequence of the gene is SEQID NO:1. The gene is used for adjusting the tobacco nicotine resultant quantity so as to adjust and control the nicotine content in tobaccos. A genetic mechanism of adjusting and controlling nicotine synthesis by degrading miRNA(Nta-miRNAX27a) of the target nicotine synthesis key gene (QPT2) through the tobacco eTM gene (Nta-eTM27) is disclosed. According to the eTM adjusting and controlling mechanism, a more-deep comprehension is provided for a role played by a key gene in the tobacco nicotine synthesis way, and the tobacco nicotine content character can be better improved conveniently.

Description

technical field [0001] The invention belongs to the field of gene technology and botany; the invention relates to a long non-coding RNA gene related to the regulation of tobacco nicotine synthesis, which functions as an endogenous target mimic (Endogenous Target Mimics, eTM) of microRNA, and participates in the synthesis of tobacco nicotine regulation. Background technique [0002] The synthesis and transport of tobacco nicotine are regulated by many factors, but at the molecular level, the research on the anabolic pathway and transport of nicotine has not been completely thorough. So far, although some key genes in the nicotine synthesis pathway have been identified and cloned, such as QPT, PMT, MPO, etc. (Dwey and Xie, 2013, Phytochemistry; Sierro et al., 2014, Nature Communication), whether these genes will be The regulation of microRNA (miRNA) pathway to affect the synthesis of nicotine and the content of nicotine is not very clear. [0003] The endogenous target mimic...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 樊龙江王卫娣李方方沈恩惠叶楚玉
Owner ZHEJIANG UNIV
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