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Novel soybean or leguminous forage rhizosphere biological-bacterial fertilizer as well as preparation method and application thereof

A technology of biological bacterial fertilizer and leguminous forage, applied in the field of fertilizer, can solve the problems of low production cost, poor environmental protection, large consumption of non-renewable resources and the like

Inactive Publication Date: 2015-04-08
GUIZHOU INST OF PRATACULTURE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to aim at the high production cost of chemical fertilizers, low fertilizer efficiency, large consumption of non-renewable resources, harm to humans and animals, and poor environmental protection in the prior art, and there are few varieties in the biological bacterial fertilizer of leguminous crops, the strain has a single function, and has a growth-promoting effect low, and the soybean crops widely planted in our country lack the specificity of biological bacterial fertilizer and other problems, a kind of biological bacterial fertilizer formula and its preparation method and application for soybean and leguminous forage are proposed to realize the low cost of fertilizer production, The consumption of non-renewable resources is small, and at the same time, it has the functions of dissolving phosphorus, fixing nitrogen, and secreting plant hormones. The purpose of good effects of promoting growth, increasing production and increasing quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1: the preparation of biological bacterial fertilizer

[0046]Inoculate 6 excellent phosphate-dissolving strains DR19, DR9, and DR24, excellent plant hormone-secreting strain DR4, and excellent rhizobium strains D2 and D4, respectively, on LB solid medium, and invert overnight at 26°C. Activation culture, when clearly visible single colonies grow, pick single colonies and inoculate them in the same volume of SOC liquid medium respectively, shake culture at 26°C at the same time, when the culture medium starts to become turbid, DR19, DR9, DR24, DR4 , D2, and D4 were inoculated in the same SOC liquid medium at the inoculum volume of 1:1:1:2:1:1, at 26-28°C, 180-200rpm (rotation per minute) Mix and vibrate culture under the conditions, and stop the fermentation when the OD600 (absorbance at a wavelength of 600 nanometers) of the bacterial liquid reaches 0.5-0.8, and obtain a liquid live bacterial preparation, use humus as a carrier, and then absorb the live bact...

Embodiment 2

[0047] Example 2: The effect of biological bacterial fertilizer on soybean growth-promoting and yield-increasing

[0048] In the first ten days of June 2012, 20kg of seeds from the same batch of soybean variety "Qiandou No. 1" were selected and divided into 2 parts on average, each 10kg. Take 2 bags of the prepared biological fertilizer, add an appropriate amount of viscous rice soup to make a paste, then add one part of 10kg of seeds, stir well and mix thoroughly, and the other part of 10kg of soybeans does not use biological fertilizer as a control. Two connected test fields with the same area and the same soil environment were sown on the same day , In the same cultivation management at the same time. In mid-July, 100 bean seedlings were randomly selected in two test fields to measure the plant height and stem diameter at the branching stage, at the end of July to measure the plant height and stem diameter at the flowering stage, and at the end of August to measure the ...

Embodiment 3

[0049] Embodiment 3: the growth-promoting effect of biological bacterial fertilizer on white clover

[0050] In mid-September, 2012, 2.0kg of seeds from the same batch of white clover variety "Haifa" were selected and divided into 2 parts on average, each 1.0kg. Take 1 bag of the prepared biological fertilizer, add an appropriate amount of viscous rice soup to make a paste, then add one part of 1.0kg of seeds, stir well and mix thoroughly, and the other part of 1.0kg of white clover does not need to be mixed with biological fertilizer As a control, they were sown on the same day in two connected test fields with the same area and the same soil environment. , In the same cultivation management at the same time. During the budding and flowering period in April 2013, 100 white clover plants were randomly selected in two test fields, and the plant height and aboveground biomass were measured. The biomass measurement method is: cut off the root system of 100 white clover plant...

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Abstract

The invention discloses a novel soybean or leguminous forage rhizosphere biological-bacterial fertilizer as well as a preparation method and an application thereof. The biological-bacterial fertilizer is prepared from the following strains: strain DR19, strain DR9, strain DR24, strain DR4, strain D2 and strain D4 at a bacterial liquid volume ratio of 1: 1: 1: 2: 1: 1. By applying the biological-bacterial fertilizer disclosed by the invention, insoluble phosphorus in the soil can be activated, nitrogen in the air can be fixed, the effective nitrogen and phosphorus nutrients in the soil for soybean and leguminous forage can be increased, plant growth hormone can be secreted, plants are promoted to absorb other nutrients and thus the growth of the plants is promoted, the nutrition structure of the plants is improved, the yield of soybean is increased and the nutritional quality of the forage is improved.

Description

technical field [0001] The invention relates to the field of fertilizers, in particular to a novel soybean or leguminous herbage rhizosphere biological bacterial fertilizer, a preparation method thereof and an application of the biological bacterial fertilizer. Background technique [0002] Nitrogen and phosphorus are one of the macroelements necessary for plant growth and development, but the lack of nitrogen and phosphorus in soil has become a common problem faced by many countries in the world. As far as our country is concerned, the survey results show that 74% of the cultivated land is deficient in phosphorus, and the phosphorus in the farmland soil exists in the combined state of phosphate. The phosphorus in the combined state is due to the Fe in the soil. 3 +, Ca 2 + and Al 3 + etc. are insoluble in water and are difficult to be absorbed by plants, so a large amount of soluble phosphorus fertilizer must be applied to the soil every year in agricultural production. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F11/08C05G3/00A01G1/00C12N1/20C12R1/22C12R1/41C12R1/01
CPCC12N1/20C05F11/08
Inventor 王小利曾庆飞陆瑞霞刘正书陈莹舒健虹李小冬
Owner GUIZHOU INST OF PRATACULTURE
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