Breeding method for the self-incompatible line of brassica campestris L. ssp. chinensis (L.) makino var. rosularis tsen et lee

Abstract

The invention discloses a breeding method for the self-incompatible line of brassica campestris L. ssp. chinensis (L.) makino var. rosularis tsen et lee. The breeding method comprises the steps of by adopting the self-incompatible line of the brassica campestris L. ssp. chinensis (L.) makino var. rosularis tsen et lee as a recurrent parent, and adopting the self-incompatible line of brassica chinensis as a transferring-in source, and carrying out back cross on filial generations and the recurrent parent for a plurality of generations so as to obtain the stable self-incompatible line of the brassica campestris L. ssp. chinensis (L.) makino var. rosularis tsen et lee. The breeding method disclosed by the invention has the advantages that the generations are screened by utilizing the brassica chinensis which has extremely-close genetic relationship with the brassica campestris L. ssp. chinensis (L.) makino var. rosularis tsen et lee and has rich self-incompatible materials as the transferring-in source of the self-incompatible line and combining selfing segregation, so that the purpose of breeding the self-incompatible line of the brassica campestris L. ssp. chinensis (L.) makino var. rosularis tsen et lee in a high-efficiency and fast manner is achieved; compared with the conventional method, the breeding method has the advantages that the working blindness is greatly alleviated, the time and the labor are saved, simultaneously the parent-material source is wide and the technical problem of difficult variety breeding for the self-incompatible line of the brassica campestris L. ssp. chinensis (L.) makino var. rosularis tsen et lee is overcome.

Description

technical field [0001] The invention relates to the technical field of crop genetics and breeding, in particular to a method for breeding self-incompatible lines of Utah cabbage. Background technique [0002] At present, there are mainly two ways to obtain self-incompatible lines on cruciferous vegetables: 1) self-segregation method (Du Min, Breeding and utilization of self-incompatible lines of cruciferous vegetables, Chinese species Ye, 2005), that is, self-incompatible strains are selected by selfing at the flowering stage and budding stage, and then self-incompatible lines are selected by mixed pollen pollination in the offspring. This method has a large workload and a long cycle. , the efficiency is low; 2) intra-species hybridization method, such as Ma Chaozhi, Fu Tingdong, Gao Changbin et al. provided "a kind of breeding method of Brassica napus self-incompatibility two-line hybrid", this method uses Brassica napus The self-incompatible line S-1300 was used as the fe...

AI Technical Summary

Problems solved by technology

[0002] At present, there are mainly two ways to obtain self-incompatible lines on cruciferous vegetables: 1) self-segregation method (Du Min, Breeding and utilization of self-incompatible lines of cruciferous vegetables, Chinese species Ye, 2005), that is, self-incompatible strains are selected by selfing at the flowering stage and budding stage, and then self-incompatible lines are selected by mixed pollen pollination in the offspring. This method has a large workload and a long cycle. , the efficiency is low; 2) intra-species hybridization method, such as Ma Chaozhi, Fu Tingdong, Gao Changbin et al. provided "a kind of breeding method of Brassica napus self-incompatibility two-line hybrid", this method uses Brassica napus The self-incompatible line S-1300 was used as the female parent, and it was crossed with the self-compatible Brassica napus line Huashuang 3 to obtain F1 seeds; The incompatible plants are self-seeded by peeling buds to obtain F2 plants, and the compatibility of the F2 plants is detected, and the incompatible plants are selected for artificial peeling and self-crossing to obtain F3 seeds; F3 seeds are sown, F3 families are selected, and the selected plants are selected All individual plants of the line were bagged and self-bred, and 3 to 5 individual plants were selected for each line to carry out artificial peeling and self-seed to obtain F4 seeds; seeds harvested from F3 lines with individual plant affinity indexes less than 1 were sown, Select the F4 family, bagging and selfing all individual plants of the selected strains, select 3 to 5 individual plants for self-breeding by artificial peeling, and select F4 families with affinity indices less than 1 as the required Brassica napus Self-incompatibility line; to the F3 family line with unstable self-incompatibility, repeat the above-mentioned selection steps until the self-incompatibility line of Brassica napus with stable self-incompatibility is selected; the method is The easiest way to create and propagate self-incompatibility materials, but with the depletion of genetic resources within a single plant species, this method is limited to varieties, and the range of self-incompatibility transfer is narrow

[0003] Utah is a variety of Brassica Brassica Brassica subspecies. So far, there has been no report that self-incompatible plants of Utah have been found in cultivation experiments, regardless of whether self-segregation is used. It is difficult to obtain the self-incompatibility line varieties of Wutaicai by either the intra-species hybridization method or the inter-species hybridization method.

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