A composite process for extracting tea seed oil by aqueous enzymatic method
A technology of tea seed oil and composite process, which is applied in the production of fat oil/fat, and fat production, can solve the problems of time-consuming and laborious, solvent residue, low yield of clear oil, etc., so as to reduce production process and production time, and avoid organic solvents. Residual, effect of simplified extraction procedure
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Embodiment 1
[0092] A composite process for extracting tea seed oil by aqueous enzymatic method, the steps are as follows:
[0093] (1) Remove the tea seeds, crush them, pass through a 60-mesh sieve, and fry them at a constant temperature of 100°C for 20 minutes, and store them in airtight storage for later use;
[0094] (2) Weigh the roasted tea seed powder, add some distilled water and Na 2 CO 3 , NaCl mixed salt solution, fully stirred and mixed, adjust the pH to 9; the reaction solution is Na 2 CO 3 , NaCl, alkaline protease mixed aqueous solution, in which Na 2 CO 3 The concentration ratio of NaCl and NaCl is 0.4mol / L:0.4mol / L, and it is dissolved in some distilled water to make a salt solution before adding;
[0095] (3) Warm up the mixed tea seed powder solution to 55°C, then add the prepared enzyme solution under stirring conditions, and close the stirring reaction for 3.5 hours; the enzyme solution is prepared by dissolving alkaline protease in partially distilled water , th...
Embodiment 2
[0098] A composite process for extracting tea seed oil by aqueous enzymatic method, the steps are as follows:
[0099] (1) Shell the tea seeds, crush them, pass through a 60-mesh sieve, and fry at a constant temperature of 100°C for 15 minutes, and set aside;
[0100] (2) Weigh the roasted tea seed powder, add some distilled water and Na 2 CO 3 , NaCl mixed salt solution, fully stirred and mixed; the reaction solution is Na 2 CO 3 , NaCl, alkaline protease mixed aqueous solution, in which Na 2 CO 3 The concentration ratio of NaCl and NaCl is 0.2mol / L:0.8mol / L, and it is dissolved in some distilled water to make a salt solution before adding;
[0101] (3) Warm up the mixed tea seed powder solution to 52°C, then add the prepared enzyme solution under stirring conditions, and stir for 4 hours; the enzyme solution is prepared by dissolving alkaline protease in partially distilled water, alkaline The dosage of protease is 1.68g / KG tea seed powder;
[0102] (4) After the reac...
Embodiment 3
[0104] A composite process for extracting tea seed oil by aqueous enzymatic method, the steps are as follows:
[0105] (1) Remove the tea seeds, crush them, pass through a 60-mesh sieve, and fry them at a constant temperature of 100°C for 30 minutes, and store them in airtight storage for later use;
[0106] (2) Weigh the roasted tea seed powder, add some distilled water and Na 2 CO 3 , NaCl mixed salt solution, fully stirred and mixed, adjust the pH to 8.5; the reaction solution is Na 2 CO 3 , NaCl, alkaline protease mixed aqueous solution, in which Na 2 CO 3 The concentration ratio of NaCl and NaCl is 0.3mol / L:0.6mol / L, and it is dissolved in some distilled water to make a salt solution before adding;
[0107] (3) Warm up the mixed tea seed powder solution to 57°C, then add the prepared enzyme solution under stirring conditions, and close the stirring reaction for 3 hours; the enzyme solution is prepared by dissolving alkaline protease in partially distilled water, The...
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