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Application of human nectin‑2 protein

A protein and fusion protein technology, applied in the application field of human Nectin-2 protein, can solve the problem of genetic distance being close to these genes, and achieve the effects of enhancing insulin sensitivity, reducing blood sugar, and improving glucose tolerance

Active Publication Date: 2017-07-18
BEIJING VDJBIO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In previous reports, there are many studies on the genes related to hyperlipidemia, such as ApoE, ApoC1 and Tomm40, and these genes are located on the same chromosome, and the genetic distance is close to these genes, and they are highly expressed in brown fat. The study on the function of Nectin-2 on fat metabolism has not been reported yet

Method used

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  • Application of human nectin‑2 protein
  • Application of human nectin‑2 protein
  • Application of human nectin‑2 protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033]Example 1 Obtaining of human Nectin-2 protein

[0034] According to the human Nectin-2 mRNA sequence published in GenBank (Accession No.: CR456818.1), the 1-1035th nucleotide sequence was spliced ​​with the gene sequence of the immunoglobulin Fc fragment. The spliced ​​gene sequence is shown in SEQ ID No.5 is shown.

[0035] After the above sequence was artificially synthesized, it was cloned into the mammalian expression vector pcDNA3.1, and the constructed expression vector was transiently transferred into HEK293 cells to realize the expression of the fusion protein and purify the target protein. The results of SDS-PAGE gel electrophoresis after protein purification are as follows: figure 2 shown.

[0036] The specific process of cell transfection, cell culture and protein purification is as follows: First, HEK293 cells were cultured in suspension in 293FreeStyle medium (GIBCO) in 5% CO 2 Cultivate under conditions, the speed of the carbon dioxide shaker (INFORS) i...

Embodiment 2

[0037] Example 2 Application of Human Nectin-2 Protein in Suppressing Obesity

[0038] In order to verify the anti-obesity effect of the Nectin-2 protein prepared in Example 1, the following high-fat diet-induced obesity mouse model was constructed in this example:

[0039] 6-week-old C57BL / 6J male mice were fed with 60Kcal% high-fat diet (Research diet, D12451), and body weight was recorded every week. After 8 weeks of induction and waiting for their obesity, the high-fat diet-induced obesity group was randomly divided into a control group (IgG, 10) and an experimental group (Nectin-2, 10); then the experimental group was treated with Nectin-2 (Example 1) treated with subcutaneous injection at a dose of 5 mg / kg body weight / week; and the control group was treated with the same dose of IgG (immunoglobulin Fc as the control protein), administered by subcutaneous injection, administered weekly 2 weeks before the administration, the average daily food intake of the animals in eac...

Embodiment 3

[0041] Example 3 Application of Human Nectin-2 Protein in Lowering Fasting Blood Glucose

[0042] Existing research results have shown that obesity is often accompanied by severe insulin resistance. In the present embodiment, the following experiments were further carried out on the mice administered in the 9th week in Example 2:

[0043] After 9 weeks of administration in Example 2, the mice were fasted overnight to measure the fasting blood glucose level.

[0044] The result is as Figure 4 As shown, the fasting blood glucose (5.84mmol) of the control group (IgG) induced by high-fat diet was significantly higher than that of the experimental group treated with Nectin-2 (5.2mmol, p<0.05).

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Abstract

The present invention relates to a new application of human Nectin-2 protein. Nectin‑2 protein and its derivative protein or its modified protein can effectively inhibit obesity and diabetes, or be used for the treatment of diseases related to obesity, including but not limited to hypertension, hyperlipidemia and the like. Nectin‑2 can not only lower blood sugar, but also increase the body’s energy metabolism, improve the body’s glucose metabolism, enhance the tissue’s insulin sensitivity, and enhance the body’s adaptability to cold stimulation by activating the body’s fatty acid metabolism. And the treatment of related diseases has opened up new avenues.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of human Nectin-2 protein. Background technique [0002] The occurrence of obesity is the result of the body's energy metabolism disorder, that is, when the body's energy intake exceeds energy consumption, the remaining energy is almost completely converted into fat and stored in the white fat of the whole body. Obesity is not only an independent disease, but also a predisposing factor for type Ⅱ diabetes, cardiovascular disease, hypertension, stroke and various cancers. Obesity is showing an explosive trend worldwide. At present, there are more than 270 million overweight people in my country, and more than 96 million obese people. Obesity induces many diseases including: type Ⅱ diabetes, non-alcoholic fatty liver, gallstones, cardiovascular disease and Alzheimer's disease, and some are even the main predisposing factors of cancer. Obesity refers to a certain degree ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/46C12N15/13C12N1/19C12N1/15C12N1/21C12N5/10A61K39/395A61P3/04A61P3/10A61P43/00
CPCA61K38/00A61P3/04A61P3/10A61P43/00C07K14/70503C07K2319/30
Inventor 李自强
Owner BEIJING VDJBIO
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