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Method for extracting L-citrulline employing microbial fermentation of trichosanthes kirilowii maxim pulp

A microbial fermentation, Trichosanthes crumb technology, applied in the direction of fermentation, preparation of urea derivatives, chemical instruments and methods, etc., can solve the problems of low conversion rate of L-arginine, unfriendly environment, complicated process, etc., and achieve obvious Safety advantages, good product properties, and high purity effects

Inactive Publication Date: 2015-03-18
安阳天尊生物工程股份有限公司
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Problems solved by technology

L-citrulline produced by fermentation and enzymatic methods is currently the main production method of L-citrulline in the market. These methods all have problems such as low conversion rate of L-arginine in pulp, complicated process, and unfriendly environment. defect

Method used

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  • Method for extracting L-citrulline employing microbial fermentation of trichosanthes kirilowii maxim pulp

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Experimental program
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Embodiment

[0030] 1. Cutting: Wash 2 kg of fresh Trichosanthes with water, and then chop them with a shredder;

[0031] 2. Separation of skin, pulp and seeds: use glue stick soft separator and two separation units to perform primary and secondary separation, so that the skin, pulp and seeds are completely separated;

[0032] 3. Fermentation: Inject 0.2%-0.3% Lactobacillus into the flesh of Trichosanthes chinensis, and ferment in airtight for 18 days;

[0033] 4. Separation: After fermentation, heat up to 70°C, sterilize for 1.5 hours, and filter;

[0034] 5. Clarification: Combine the filtrates and pass through a 0.2 micron microfiltration membrane and a 5000 molecular weight ultrafiltration membrane for clarification. The microfiltration membrane can be 4.3'SuperCorHFM180 tubular membrane element produced by KOCH, and the ultrafiltration membrane can be 8338 roll membrane. element, the feed flow rate is 100 liters per minute, and the filtration pressure is 1.5 MPa. Solid insolubles an...

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Abstract

The invention discloses a method for extracting L-citrulline employing microbial fermentation of trichosanthes kirilowii maxim pulp, and belongs to the technical field of chemistry of traditional Chinese medicine. The method comprises the following steps: inoculating lactic acid bacteria to ferment; filtering; carrying out adsorption and exchange; concentrating; and crystallizing to obtain the L-citrulline. According to the method, by combination of fermentation method and an enzyme method, L-arginine in the trichosanthes kirilowii maxim pulp is converted into the L-citrulline under the action of deiminase which is generated by lactobacillus, so that the yield is greatly improved; no harmful solvent is added in the overall separation process; the obtained product is natural and safe; and development of health care products and development and research of medicines are facilitated.

Description

technical field [0001] The invention relates to a method for extracting L-citrulline, in particular to a method for extracting L-citrulline from Trichosanthes chinensis, belonging to the technical field of traditional Chinese medicine chemistry. Background technique [0002] Citrulline, first found in wild watermelon leaves, is a non-essential amino acid. L-citrulline has a strong antioxidant effect and can effectively remove free radicals in the human body. L-citrulline in watermelon is known as "green Viagra", which can effectively increase male libido, promote male reproductive capacity, and maintain normal male reproductive health. Citrulline also has a vasodilator effect and has a good health care effect on the cardiovascular system. In addition, citrulline can effectively improve the anti-fatigue ability of the human body, enhance the muscle strength of the human body, improve physical fitness, and has a good effect on sports health care. At present, foreign countri...

Claims

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Application Information

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IPC IPC(8): C07C275/16C07C273/18C12P13/10
Inventor 杨保成谢书轩
Owner 安阳天尊生物工程股份有限公司
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