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Microplate analysis method for time toxicity of environmental pollutants on basis of chlorella pyrenoidosa

A technology for Chlorella pyrenoidosa and environmental pollutants, applied in the direction of color/spectral characteristic measurement, etc., to achieve a reasonable evaluation effect

Inactive Publication Date: 2015-03-04
ANHUI UNIVERSITY OF ARCHITECTURE
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] The present invention proposes a microplate analysis method for the time toxicity of environmental pollutants based on Chlorella pyrenoidosa. Compared with the traditional toxicity analysis method, the time factor is emphasized, and the traditional toxicity analysis method only analyzes the effect of pollutants on exposed organisms in a certain time. Insufficiency of the cumulative effect of the end point of the exposure time is helpful for a comprehensive understanding of the toxic effects of pollutants, and a deeper understanding of the toxicological mechanism and pathways of pollutants, which can better reflect the phenomenon of pollutant exposure in the actual environment and information

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  • Microplate analysis method for time toxicity of environmental pollutants on basis of chlorella pyrenoidosa
  • Microplate analysis method for time toxicity of environmental pollutants on basis of chlorella pyrenoidosa
  • Microplate analysis method for time toxicity of environmental pollutants on basis of chlorella pyrenoidosa

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Embodiment 1

[0029] Adopt the present invention to measure the time toxicity of antibiotic streptomycin sulfate, comprise the following steps:

[0030] S1. Inoculate the algal species of Chlorella pyrenoidosa in fresh medium to make the OD of Chlorella pyrenoidea after inoculation 690 = 0.14, placed in a light incubator for cultivation, the temperature of the light incubator is 25±1°C, and the illuminance of the light incubator is 5000 lux, and the OD is obtained. 690 = 0.35 Chlorella pyrenoidosa liquid;

[0031] S2, refer to figure 1 , figure 1 It is a schematic diagram of 96-well transparent microplate grouping in a kind of microplate analysis method of environmental pollutant time toxicity based on Chlorella pyrenoidosa proposed by the present invention. Take 3 96-well transparent microplates with 8 rows×12 columns; In the plate, select the outermost 36 microwells and define them as the first microwells, add 200 μL of distilled water to each of the 36 first microwells, select the col...

Embodiment 2

[0040] Adopt the time toxicity of the time toxicity of the present invention to measure ionic liquid 1-octyl imidazolium chloride, comprise the following steps:

[0041] S1, insert the algal species of Chlorella pyrenoidosa in fresh medium, make the OD of Chlorella pyrenoidosa after inoculation 690 =0.16, placed in a light incubator for cultivation, the temperature of the light incubator is 25±1°C, the illuminance of the light incubator is 5000lux, and the OD is obtained 690 =0.25 Chlorella pyrenoidosa liquid;

[0042] S2, refer to figure 1 , figure 1 It is a schematic diagram of 96-well transparent microplate grouping in a kind of microplate analysis method of environmental pollutant time toxicity based on Chlorella pyrenoidosa proposed by the present invention. Take 3 96-well transparent microplates with 8 rows×12 columns; In the plate, select the outermost 36 microwells and define them as the first microwells, add 200 μL of distilled water to each of the 36 first micro...

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Abstract

The invention discloses a microplate analysis method for the time toxicity of environmental pollutants on the basis of chlorella pyrenoidosa. The microplate analysis method comprises the following steps: with chlorella pyrenoidosa in freshwater green algae as a tested organism and a 96-hole transparent microplate as a carrier, introducing a time factor into a traditional compound concentration-effect relation, determining the time dependent toxicity of partial environmental pollutants on the chlorella pyrenoidosa, thereby discovering that all the determined pollutants have obvious time dependent characteristic, and then establishing a concentration-time-effect curve surface by utilizing a least-square spline function approximation method, thereby knowing the dynamic toxicity effect of the pollutants along with change of the concentration and the time. The microplate analysis method disclosed by the invention has the advantages that the defect that a traditional toxicity analysis method can only analyze the accumulation effect of pollutants for exposed organisms at a certain exposure time endpoint is overcome, the toxicity effect of the pollutants can be learned comprehensively, the mechanism and the means of the toxicological effect of the pollutants can be known deeply, and further, the phenomenon and information of exposure of the pollutants in actual environments can be embodied.

Description

technical field [0001] The invention relates to the technical field of environmental pollutant toxicity detection, in particular to a microplate analysis method of environmental pollutant time toxicity based on Chlorella pyrenoidosa. Background technique [0002] At present, the production and life of human beings continuously discharge a large variety of pollutants into the environment, which have a continuous toxic effect on the ecological environment and organisms. Therefore, in addition to considering the concentration factor, the detection of pollutant toxicity should focus on the dynamic effect of pollutants on exposed organisms over time, that is, to simultaneously observe the two factors of concentration and time in the process of changes in the toxicity of pollutants to organisms. important role. [0003] The toxic effect of pollutants in the environment on aquatic organisms is a dynamic process. At the same time, because of the metabolic transformation in the envi...

Claims

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Application Information

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IPC IPC(8): G01N21/31
Inventor 张瑾陈琼朱曙光凌琪刘磊
Owner ANHUI UNIVERSITY OF ARCHITECTURE
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