Diaphragm type sample injection device

Abstract

The invention belongs to the field of a liquid phase chromatography analysis instrument, and in particular relates to a diaphragm type sample injection device. The diaphragm sample injection device comprises an upper adaptor (1) internally provided with a cavity, a lower adaptor (2) which is matched with the upper adaptor, and a diaphragm (5) which is arranged between the upper adaptor (1) and the lower adaptor (2); and the diaphragm sample injection device also comprises a shunt (3) which is arranged in the inner cavity of the upper adaptor (1), the shape of the shunt (3) is matched with the shape of the inner cavity of the upper adaptor, a gap which is less than or equal to 2mm is formed between the shunt (3) and the inner wall of the upper adaptor, and at least one bulge is arranged in the gap to be used for connecting the shunt and the inner wall of the upper adaptor. By adopting the diaphragm type sample injection device, the diffusion factor of elution is reduced through the axial and radial transformation of an elution solution, and the sample carrying area of the diaphragm is increased.

Description

technical field [0001] The invention belongs to the field of liquid chromatography analysis instruments, and in particular relates to a membrane type sampling device. Background technique [0002] In liquid chromatography analysis, the sample injection method is usually to inject the sample solution into the quantitative loop and connect it to the liquid chromatography system through a switching valve, so the sample must be in a solution state before it can be injected. However, many substances are prone to decomposition, adsorption and conversion in solution, which is not conducive to long-term storage and storage. Therefore, a liquid chromatography sampling method that is conducive to stable storage of samples is needed. [0003] There is a literature report on a dry blood spot (dried blood spot, DBS) technology combined with liquid chromatography, the process is to drop the sample (such as blood, biological fluid, etc.) on a circular paper, The sample is evaporated or b...

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Problems solved by technology

[0004] There is such a shortcoming in the above-mentioned tight-fitting system: because the eluting solution flows through the paper from the direction perpendicular to the plane of the paper in the said tight-fitting system, and the caliber of the eluting solution inlet is much smaller than the diameter of the paper, so The farther the distance from the center of the paper, the smaller the flow velocity of the eluting solution. Therefore, the samples distributed on the paper farther from the center will take a longer time for the target components to be eluted. The samples closer to the center, the target Components take a short time to elute, resulting in severe dispersion of target elution peaks

The current solution is to reduce the area of ​​the membrane, reduce the path for the eluting solution to diffuse to the edge, and allow the nearer and farther samples to be eluted within a tolerable time range, but too small a paper area will limit the sample. sample load

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