Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Latex particles for agglutination assay

A technology of latex particles and hydrogen atoms, which is applied to measuring devices, instruments, and analytical materials, and can solve problems such as carrier particles that cannot be used as diagnostic agents

Active Publication Date: 2015-02-04
SEKISUI MEDICAL CO LTD
View PDF7 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, a high content of the compound represented by this formula is used in the preparation of polymer particles; therefore, the resulting polymer particles hardly physically adsorb antigens or antibodies onto their surfaces and cannot function as carrier particles for diagnostic agents. effect

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Latex particles for agglutination assay
  • Latex particles for agglutination assay

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Ultrapure water (1000g), styrene monomer (135g), Blemmer PE-90 (R 1 = methyl, R 2 =H, n=2, available from NOF CORPORATION) (0.24g), sodium styrene sulfonate (1.2g), and potassium persulfate (0.7g) were placed in a device equipped with a stirrer, reflux cooler, temperature detector , a nitrogen inlet tube, and a jacketed glass reactor (volume: 2 L). After purging the vessel with nitrogen, the mixed solution was polymerized at 70° C. for 24 hours with stirring at 210 rpm.

[0086] After stopping the polymerization, the solution was filtered through filter paper to extract latex particles. The latex particles were dialyzed for 48 hours through a dialysis membrane to refine the latex particles. The latex particles have a particle diameter of 0.109 μm (CV: 8.4%) and 0.192 μmol / m 2 PEG density.

Embodiment 2

[0088] Except for Blemmer PE-200(R 1 = A tomb, R 2 =H, n=4 to 5, available from NOF CORPORATION) (0.39g) instead of Blemmer PE-90 (R 1 = methyl, R 2 =H, n=2, available from NOF CORPORATION) (0.24 g), latex particles were prepared as in Example 1. The latex particles have a particle diameter of 0.108 μm (CV: 11.7%) and 0.191 μmol / m 2 PEG density.

Embodiment 3

[0090] Except for Blemmer PE-350(R 1 = methyl, R 2 =H, n=8, available from NOF CORPORATION) (0.60g) instead of Blemmer PE-90 (R 1 = methyl, R 2 =H, n=2, available from NOF CORPORATION) (0.24 g), latex particles were prepared as in Example 1. The latex particles have a particle diameter of 0.106 μm (CV: 9.8%) and 0.187 μmol / m 2 PEG density.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Particle sizeaaaaaaaaaa
Particle sizeaaaaaaaaaa
Login to View More

Abstract

A latex particle for high-sensitive agglutination assay and a reagent for agglutination assay including the particle are provided. The latex particle barely initiates non-specific reactions and can readily prepare diagnostic agents. A latex particle for agglutination assay including a polymerizable monomer having a phenyl group, a polymerizable monomer having a phenyl group and a salt of sulfonic acid, and a polymerizable monomer represented by Formula (1): €ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒCH 2 =CR 1 -COO (CH 2 CH 2 O) n -R 2 €ƒ€ƒ€ƒ€ƒ€ƒ(1) where R 1 represents a hydrogen atom or a methyl group; R 2 represents a hydrogen atom or a methyl group; and n is 1‰¤n<20, wherein the density of functional groups derived from the polymerizable monomer represented by Formula (1) on the surface of the particle is 0.05 to 0.5 µmol / m 2 .

Description

technical field [0001] The present invention relates to latex particles for highly sensitive agglutination measurement which hardly cause non-specific reactions, and a reagent for agglutination measurement using the latex particles. Background technique [0002] In the field of clinical examinations, immunoassays using antigen-antibody reactions have been widely performed to determine trace substances in samples. Among these, latex immunoturbidity assay using antibody-carrying latex particles (hereinafter also referred to as sensitized latex particles) has been widely used in laboratories because latex immunoturbidity can be achieved by simple operation in a short time. Determination. In latex immunoturbidimetric assays, the amount of antigen or antibody in a sample is determined by optical detection of changes in absorbance resulting from agglutination of sensitized latex particles during immune complex formation. This change in absorbance is based on the apparent change ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/545C08F212/08C08F212/14C08F220/28G01N33/543
CPCC08F212/14G01N33/585C08F212/08C08F220/28C08F220/286C08F212/30
Inventor 北原慎一郎高桥由纪
Owner SEKISUI MEDICAL CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com