Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Aquaporin gene specifically expressed by virtue of chorispora bungeana root as well as protein encoded by aquaporin gene and method for cloning aquaporin gene

An aquaporin, gene-encoded technology, applied in the field of plant stress resistance gene research to achieve the effect of enhancing stress resistance

Inactive Publication Date: 2015-02-04
李鸣
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no relevant report on the alpine ion mustard. Therefore, the cloning of the alpine ion mustard aquaporin gene and the study of its expression will help to clarify the molecular mechanism of the action of the aquaporin gene, and at the same time obtain information that can be applied to other tropical plants. Gene resources for improving frost resistance of cold-sensitive plants in subtropical regions

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Aquaporin gene specifically expressed by virtue of chorispora bungeana root as well as protein encoded by aquaporin gene and method for cloning aquaporin gene
  • Aquaporin gene specifically expressed by virtue of chorispora bungeana root as well as protein encoded by aquaporin gene and method for cloning aquaporin gene
  • Aquaporin gene specifically expressed by virtue of chorispora bungeana root as well as protein encoded by aquaporin gene and method for cloning aquaporin gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1) Extraction and detection of total RNA from alpine mustard root

[0023] According to the method of using Trizol reagent from Invitrogen Company, total RNA was extracted from Alpine mustard root and reverse-transcribed into cDNA according to the method of using M-MLV Reverse Transcriptase (TakaRa) and stored at -20°C for future use.

[0024] 2) Acquisition of the first sequence of the aquaporin gene ChlRB7 specifically expressed in the root of alpine ionic mustard

[0025] Download the cloned related genes such as tobacco, carrot and sunflower gene sequences from Genbank, and perform sequence comparison. According to the comparison results, degenerate primers were designed in the conserved region of CDS, and two bands of about 750bp and 391bp in size were obtained after PCR amplification using the indirect primers. Among them, the 391bp fragment was consistent with the expected result, and the sequence alignment of the T-clone sequencing results on NCBI found that th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an aquaporin gene specifically expressed by virtue of chorispora bungeana root as well as a protein encoded by the aquaporin gene and a method for cloning the aquaporin gene and belongs to the field of research on plant-resistant genes. The aquaporin gene specifically expressed by virtue of chorispora bungeana root disclosed by the invention is derived from chorispora bungeana; and under the condition of low-temperature stress, the expression quantity of the aquaporin gene in chorispora bungeana is significantly increased and the stress resistance of chorispora bungeana is enhanced due to the over-expression of the gene.

Description

technical field [0001] The invention relates to an aquaporin gene specifically expressed in alpine mustard root, its coded protein and its gene cloning method, and belongs to the field of plant stress resistance gene research. Background technique [0002] Low temperature stress often makes some plants originating in tropical and subtropical regions unable to overwinter safely in the open field in the north of the temperate zone due to cold sensitivity, and also brings serious disasters to tropical and subtropical plants due to occasional low temperature stress. Therefore, modifying the genetic characteristics of crops to increase cold resistance has become a long-term pursuit of plant breeders. Unfortunately, traditional breeding methods have made little progress in improving crop cold resistance. It can be seen from the development trend of modern biotechnology and molecular biology that transgenic technology is one of the most effective ways to solve this problem. Chill...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/29C12N15/10C07K14/415
Inventor 李鸣高天鹏张勇晋玲刘光琇安黎哲
Owner 李鸣
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com