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Clam bioactive peptide and its extraction method and use

A bioactive peptide, clam biotechnology, applied in application, peptide, food science and other directions, can solve the problem of insignificant enhancement of macrophage phagocytic activity and spleen lymphocyte proliferation ability, and achieve enhanced phagocytic neutral red ability. , the effect of enhancing immunity and promoting the ability of proliferation

Inactive Publication Date: 2015-01-28
GUANGXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of this, the purpose of the present invention is to propose a clam biologically active peptide and its extraction method and application to overcome the insignificant effect of existing marine biological polypeptides on the macrophage phagocytosis activity of the immune system and the proliferation of spleen lymphocytes. Defects

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  • Clam bioactive peptide and its extraction method and use
  • Clam bioactive peptide and its extraction method and use
  • Clam bioactive peptide and its extraction method and use

Examples

Experimental program
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Effect test

Embodiment 1

[0021] The extraction method of embodiment 1 clam bioactive peptide

[0022] (1) Pretreatment: take fresh and live clams, remove the shell, take about 25.0kg of muscle tissue and mucus, and add them to an acetic acid solution with a mass concentration of 1.1%. The mass ratio of muscle tissue and mucus to acetic acid solution is 1:1 , homogenized with a high-speed tissue crusher to obtain a homogenate.

[0023] (2) Enzymolysis: Add papain, neutral protease and trypsin to the homogenate obtained in step (1), the mass ratio of papain, neutral protease and trypsin is 3:7:2, the enzymolysis temperature 42-45°C, the amount of enzyme added is 0.5% of the wet weight of clam muscle tissue and mucus, adjust the pH value of the homogenate to 7.5-7.9, heat up to 85°C after enzymolysis for 1-2 hours, and inactivate the enzyme for 10 minutes; The homogenate was centrifuged to obtain the supernatant, which was the required enzymatic hydrolysis solution, and stored at low temperature (can be...

Embodiment 2

[0067] Experimental Study on the Enhancement of Immunity by Biologically Active Peptides of Meretrix Meretrix

[0068] 1. Effect of clam bioactive peptides provided by the present invention on the ability of mouse macrophages to phagocytize neutral red

[0069] Mouse mononuclear macrophage RAW264.7 phagocytosis neutral red experiment preparation cell concentration is 1×10 6 Each / mL macrophage suspension was added to a 96-well plate, 100 μL / well, and clam bioactive peptide was added to intervene, and the final concentration of administration was 100 μg / mL, 50 μg / mL, and 25 μg / mL. Three replicate wells were set up for each concentration, and 1 μg / mL lipopolysaccharide (LPS for short, which can promote the proliferation of macrophages) was used as a positive control. The same volume of complete culture solution was used as zero well. After culturing for 48 hours, add 100 μL of 0.1% neutral red saline solution to each well, continue culturing for 30 minutes, pour off the superna...

Embodiment 3

[0090] The clam biologically active peptide is added with appropriate pharmaceutical excipients to make corresponding medicines or health food, such as capsules, tablets, granules, powders, pills, oral liquids or injections, and the medicine or health food made of the present invention is obtained.

[0091] As an example of the present invention, when adding lactose and cysteine, the mass ratio of clam biologically active peptide, lactose and cysteine ​​is about 10:9:1. Specifically, take 500.0 g of clam bioactive peptides of the present invention (purity ≥ 98.5%), mix evenly with 450.0 g of pharmaceutical grade lactose and 50.0 g of cysteine, put them into 0# capsules, and then put the capsules into medicinal white plastic The bottle is sealed, and the finished product is obtained.

[0092] As an example of the present invention, pharmaceutical grade lactose and cysteine ​​can be added, and the mass ratio of clam biologically active peptide, lactose and cysteine ​​is about 8:...

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Abstract

The invention discloses a clam bioactive peptide and its extraction method and use. The clam bioactive peptide has an N-tail end sequence of TRGIDEIQNT GGGGTRR, an isoelectric point of 8.79 and molecular weight of 18KDa. The clam bioactive peptide can promote macrophage phagocytosis activity and splenic lymphocyte propagation capability of an immunization system, has pharmacological effects of improving immunity, can be used for preparation of a drug or a health food for improving immunity and has potential of exploitation of novel immunopotentiation drugs.

Description

technical field [0001] The invention relates to the fields of biochemistry and biomedicine, in particular to a clam bioactive peptide, an extraction method and application thereof. Background technique [0002] Immunity is the defense function of the body against the invasion of pathogenic microorganisms and the function of maintaining the stability of the internal environment. The occurrence and development of many diseases are closely related to the low immune function or immunodeficiency of the body. Therefore, the development of immune drugs has become the focus of research by medical workers. [0003] Marine natural bioactive substances are the focus of modern biological research, and marine bioactive peptides are an important part of the research on marine active substances. Marine organisms can extract active peptides with various physiological functions, which have attracted wide attention due to their cheap raw materials, simple operation, and high safety. [000...

Claims

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Application Information

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IPC IPC(8): C07K7/08C12P21/06A61K38/10A61P37/02A23L1/305A23L33/18
Inventor 杜正彩邓家刚郝二伟吴玉强
Owner GUANGXI UNIV OF CHINESE MEDICINE
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