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Corn fiber gel-protein emulsifier and preparation method thereof

A corn fiber glue and protein technology, which is used in vegetable protein processing, food ingredients as emulsifiers, food preparation, etc., can solve the problems of unstable emulsification properties of corn fiber glue, and achieve simple processing, improved emulsification properties, and high efficiency. Effect

Inactive Publication Date: 2015-01-28
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a kind of corn fiber latex emulsifier and preparation method thereof, solve the deficiency that the emulsification properties of corn fiber latex from different sources are not stable, thereby improve the emulsification of corn fiber latex

Method used

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  • Corn fiber gel-protein emulsifier and preparation method thereof
  • Corn fiber gel-protein emulsifier and preparation method thereof
  • Corn fiber gel-protein emulsifier and preparation method thereof

Examples

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preparation example Construction

[0042] The preparation principle of the emulsifier in the present invention is as follows: in the enzymatic reaction system, corn fiber gum and protein form intermolecular copolymers to increase the protein content of the corn fiber gum and improve the emulsification of the corn fiber latex.

[0043] The preparation method of emulsifying agent of the present invention, concrete steps are as follows:

[0044] a) Prepare buffer: select buffer as the solution of the reaction system, the pH value is between 6.0 and 7.0, and the buffer is preferably phosphate buffer;

[0045] b) Preparation of corn fiber gum solution: when the corn fiber gum is prepared into a solution, select the prepared buffer solution in step a as a solvent, prepare a clear solution according to the mass fraction of the corn fiber gum between 0.125% and 5.0%, and stir at the same time until fully dissolved;

[0046] c) Preparation of protein solution: When preparing protein into a solution, select the buffer p...

Embodiment 1

[0053] The emulsifier of Example 1 has a composition: the ratio of corn fiber gum to protein is 1:4 (by mass ratio). Concrete preparation process is as follows:

[0054] a) Prepare phosphate buffered saline: prepare phosphate buffered saline (PBS) with pH 6.0;

[0055] b) Preparation of corn fiber glue solution: its mass fraction is 0.125%, and the phosphate buffer solution prepared in step a is selected as a solvent to prepare a clear solution, and stirred until fully dissolved at the same time, and the stirring time is 2 hours;

[0056] c) Preparation of bovine serum albumin solution: its mass fraction is 2.0%, and the phosphate buffer solution prepared in step a is selected as a solvent to prepare a clear solution, and stirred until fully dissolved at the same time, and the stirring time is 4 hours;

[0057] d) Preparation of H 2 o 2 And horseradish peroxidase solution: get 20 μ L of 30% (mass fraction) H 2 o 2 , diluted to 10 mL of 0.06% (mass fraction) H with the pre...

Embodiment 2

[0063] The emulsifier of Example 2 has a composition: the ratio of corn fiber gum to protein is 1:1 (by mass ratio). Concrete preparation process is as follows:

[0064] a) Prepare phosphate buffered saline: prepare phosphate buffered saline (PBS) with pH 6.0;

[0065] b) Preparation of corn fiber glue solution: its mass fraction is 0.5%, and the phosphate buffer solution prepared in step a is selected as a solvent to prepare a clear solution, and stirred until fully dissolved at the same time, and the stirring time is 2 hours;

[0066] c) Preparation of bovine serum albumin solution: its mass fraction is 2.0%, and the phosphate buffer solution prepared in step a is selected as a solvent to prepare a clear solution, and stirred until fully dissolved at the same time, and the stirring time is 4 hours;

[0067] d) Preparation of H 2 o 2 And horseradish peroxidase solution: get 20 μ L of 30% (mass fraction) H 2 o 2 , diluted to 10 mL of 0.06% (mass fraction) H with the prepa...

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Abstract

The invention belongs to the technical field of food processing, and in particular relates to a corn fiber gel-protein emulsifier and a preparation method thereof. The emulsifier comprises corn fiber gel and protein in a ratio of 0.25-10 (in mass ratio). The preparation method comprises the following steps: preparing a buffer liquid, preparing a corn fiber gel solution, preparing a protein solution, preparing a H2O2 and peroxidase solution, preparing a corn fiber gel and protein copolymer, standing, performing enzyme deactivation, concentrating and freeze-drying. The emulsifier is simple and convenient in processing and high in efficiency, the emulsification property of the corn fiber gel under different environment conditions (pH value / ion strength / temperature) is effectively improved, and meanwhile the copolymer of the corn fiber gel-protein is not remarkably changed in color and is free of adverse odor.

Description

technical field [0001] The invention belongs to the technical field of food processing, and in particular relates to a corn fiber glue-protein emulsifier and a preparation method thereof. Background technique [0002] Corn fiber gum is a kind of hemicellulose extracted from the by-products of corn milling by alkali extraction-alcohol precipitation. Corn fiber gum is mainly composed of xylan, and contains a small amount of protein and ferulic acid. As a natural polysaccharide-protein complex, it can be used in the preparation of oil-in-water emulsifiers. However, the chemical composition of corn fiber gum prepared from different raw materials is quite different, which directly affects the quality of its emulsifying performance, which becomes one of the key factors that limit the wide application of corn fiber gum. [0003] The combination of polysaccharides and proteins is a common method to improve the emulsification of polysaccharides. There are usually two ways of bindi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/10A23J3/14A23J3/34A23L7/10
CPCA23V2002/00A23V2200/222
Inventor 殷丽君刘妍邱爽庞晓晗
Owner CHINA AGRI UNIV
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