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Bacillus megaterium, application of bacillus megaterium, microbial degradation agent and preparation method and application of microbial degradation agent

A Bacillus megaterium and microbial degradation technology, applied in the field of microorganisms, can solve the problems of limiting the practical application of triazophos microbial restoration technology, the safety of degrading bacteria resources, and the lack of screening of degrading bacteria resources, and achieves low production cost and wide application. Value, good removal effect

Inactive Publication Date: 2015-01-21
HUNAN PLANT PROTECTION INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Phytoremediation technology has the advantages of less cost and environmental safety. However, due to the characteristics of plants, suitable growth conditions are required and the growth rate is low, so the restoration efficiency is low.
Although the field of triazophos microbial remediation has great development prospects, the current technical problems are: the screening of degrading bacteria resources is relatively small, and some degrading bacteria resources themselves have safety problems, which limits the application of triazophos microbial remediation technology. practical application

Method used

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  • Bacillus megaterium, application of bacillus megaterium, microbial degradation agent and preparation method and application of microbial degradation agent
  • Bacillus megaterium, application of bacillus megaterium, microbial degradation agent and preparation method and application of microbial degradation agent
  • Bacillus megaterium, application of bacillus megaterium, microbial degradation agent and preparation method and application of microbial degradation agent

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Effect test

Embodiment 1

[0032] A Bacillus megaterium TDB-2 ( Bacillus megaterium TDB-2) strain, preserved in the China Center for Type Culture Collection (CCTCC for short), the preservation number is CCTCC NO: M 2012352, the address of the preservation unit is located at Wuhan University, China, and the preservation date is September 16, 2012. The strain was named For Bacillus megaterium TDB-2 ( Bacillus megaterium TDB-2).

[0033] The aforementioned Bacillus megaterium TDB-2 has the following main characteristics:

[0034] G + , The individual shape is rod-shaped, endophytic spores, no swelling, positive contact enzyme reaction, can liquefy gelatin, hydrolyzed starch, V-P reaction is negative, indole reaction and catalase reaction are negative, glucose produces acid but does not produce gas. The optimum growth temperature is 30-35℃, the optimum growth pH value is 7-8, the length of 16S rDNA sequence is 1442 bp, and the accession number in Genbank is JX393073.

[0035] see figure 1 ,From figu...

Embodiment 2

[0042] A microbial degradation agent, comprising the bacillus megaterium TDB-2 of embodiment 1 and auxiliary materials.

[0043] The concentration of Bacillus megaterium TDB-2 in the microbial degradation agent is generally 10 8 ~10 9 cfu / mL, the concentration of Bacillus megaterium TDB-2 is 10 in the present embodiment 9 cfu / mL, excipients are commonly used excipients in water or other microbial degradation agents.

[0044] The microbial degradation agent of embodiment 2 is prepared by the following method:

[0045] S1, inoculate Bacillus megaterium TDB-2 on MSY solid medium (MSY solid medium composition: 0.2g / L K 2 HPO 4 , 0.8g / L KH 2 PO 4 , 0.2g / L MgSO 4 , 0.1g / L CaSO 4 2H 2 O, 0.0033g / L NaMoO 4 2H 2 O, 1.5g / L yeast extract, 3g / L NaCl, 15g / L agar, the pH value is 7.1), cultured at 30℃~35℃ according to the plate culture method until a single colony appeared.

[0046] S2. Take the aforementioned single colony and insert it into 150 mL of MSY liquid medium (MSY l...

Embodiment 3

[0050] Example 3: Application of Bacillus megaterium TDB-2 in degrading triazophos.

[0051] (1) Cultivate Bacillus megaterium TDB-2 in MSY liquid medium to form a bacterial liquid, and the concentration of Bacillus megaterium TDB-2 in the bacterial liquid is 10 9 cfu / mL;

[0052] (2) Add the wastewater containing triazophos into MSY liquid medium and mix to obtain a mixed solution, in which the concentration of triazophos is 50 mg / L. Inoculate the bacterial solution prepared in step (1) into the mixed solution at 1% (v / v), and carry out shaking culture at 30°C to 35°C (the shaking speed is 150 rpm, in the actual application process, the shaking speed is 100-150 rpm can be implemented) for 9 hours, sampling every 1 hour, and using gas chromatography (Agilent 6890N) to determine the content of triazophos.

[0053] Triazophos standard samples of known concentration were used for qualitative and quantitative determination before the determination, and the experimental medium...

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Abstract

The invention relates to the field of microorganism, in particular to bacillus megaterium, application of bacillus megaterium, a microbial degradation agent and a preparation method and an application of the microbial degradation agent, wherein the bacillus megaterium is bacillus megaterium TDB-2 with a preservation number of CCTCC NO: M 2012352 and can be used for degrading triazophos; the bacillus megaterium TDB-2 is used as the active ingredient of the microbial degradation agent, the preparation method of the microbial degradation agent comprises the following steps: culturing the bacillus megaterium TDB-2 according to a plating method until single colonies come out; inoculating a seed medium with the single colonies and culturing till a logarithmic phase, thus obtaining bacterial liquid; inoculating a production medium with the bacterial liquid according to an inoculation amount of 1-3% and culturing till the logarithmic phase, and packaging to obtain the microbial degradation agent. The bacillus megaterium TDB-2 provided by the invention can effectively degrade the triazophos and is efficient, non-toxic and friendly to the environment, etc.

Description

technical field [0001] The present invention relates to the technical field of microorganisms, in particular to a Bacillus megaterium and its application in degrading triazophos, and also relates to a microbial degradation agent with the Bacillus megaterium as an active ingredient, a preparation method thereof and a method for degrading triazophos Applications. Background technique [0002] Triazophos is a broad-spectrum, moderately toxic organophosphorus insecticide, which is widely used in the control of major insect pests of grain, oilseeds and vegetables in my country. The long-term and large-scale use of triazophos not only leads to the residue of triazophos in agricultural products, which poses potential dangers to human health, but also the applied triazophos pesticide is lost to the ecological environment due to factors such as rain erosion, which harms non-targets. organisms, thereby affecting the entire ecological environment. However, based on the actual agricul...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C12R1/11A62D101/04A62D101/28
CPCA62D3/02A62D2101/04A62D2101/28C12N1/20C12N1/205C12R2001/11
Inventor 张松柏刘勇张德咏罗香文谭新球彭静成飞雪程菊娥
Owner HUNAN PLANT PROTECTION INST
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