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A mutant strain of Nannochloropsis pseudochloropsis and its application

A technology of Nannochloropsis and mutant strains, applied in the field of microorganisms, can solve the problems of decreased algal cell activity, growth of polluted algal strains, and death of algal cells, and achieve strong environmental adaptability, insecticide and high temperature tolerance Strong, high-fat effect

Active Publication Date: 2017-09-15
ENN SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, in the large-scale outdoor cultivation of microalgae, it is easy to breed protozoa, bacteria and other pollution, which seriously pollutes the growth of algal strains.
Although the use of pesticides can effectively inhibit pollution, it will damage the algal strains, resulting in a decrease in the activity of algal cells and a reduction in production. When the concentration of pesticides is too high, the algal cells will die

Method used

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  • A mutant strain of Nannochloropsis pseudochloropsis and its application
  • A mutant strain of Nannochloropsis pseudochloropsis and its application
  • A mutant strain of Nannochloropsis pseudochloropsis and its application

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Experimental program
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Effect test

specific Embodiment 1

[0046] Specific embodiment 1 Screening and Obtaining of ENN11-4 Algae Strain

[0047] The sterile algae liquid of Nannochloropsis sp. was subjected to EMS mutagenesis treatment, and sodium hypochlorite insecticide and hydroxyproline were added to repeat pressure treatment, primary screening and secondary screening, and finally a mutant strain ENN11-4 with excellent performance was obtained. The mutant algae strain is preserved in the General Microorganism Center of China Microorganism Culture Collection Management Committee, and the preservation number is CGMCC No.9320.

specific Embodiment 2

[0048] Specific embodiment 2 Routine cultivation and evaluation of algae strain ENN11-4 in indoor column reactor

[0049] 1) 3cm column reactor evaluation: ENN11-4 green swimming cells in the logarithmic growth phase were inoculated in the prepared seawater medium to make the cell density reach OD 750 between 0.8-1.2, with wild-type ENN11 as the control.

[0050] During the cultivation process, the light intensity is controlled at 50-500umol / m 2 .s, L:D=16:8 light-dark cycle culture. During the culture period, the pH value of the culture medium is adjusted between 7-9 by feeding a mixed gas of 1.5-2% carbon dioxide and air into the culture solution. The reactor used for the cultivation was a column reactor with an inner diameter of 30 mm and a length of 600 mm. After culturing for 8 days, the growth curve of the algae strain was determined.

[0051] Such as figure 1 As shown, the growth of ENN11-4 cultured under conventional conditions was significantly better than th...

specific Embodiment 3

[0054] Specific embodiment 3 Evaluation of Pesticide Pressure Culture in Indoor Column Reactor of ENN11-4 Algae Strain

[0055] 1) Insecticide pressure evaluation in 3cm column reactor: ENN11-4 green swimming cells in the logarithmic growth phase were inoculated in the prepared seawater medium, so that the cell density reached OD 750 between 0.8-1.2, with wild-type ENN11 as the control. During the cultivation process, the light intensity is controlled at 50-500umol / m 2 .s, L:D=16:8 light-dark cycle culture. During the culture period, the pH value of the culture medium is adjusted between 7-9 by feeding a mixed gas of 1.5-2% carbon dioxide and air into the culture solution. The reactor used for the cultivation was a column reactor with an inner diameter of 30 mm and a length of 600 mm. Add 25 ppm of available chlorine at the beginning of the inoculation, and treat it in the dark for 8 hours, then culture it under light, and cultivate it for 7 days to measure the growth c...

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Abstract

The invention relates to a Nannochloropsis mutant strain and application thereof. The Nannochloropsis mutant strain of the invention is taxonomically named Nannochloropsis sp. The Nannochloropsis mutant strain of the invention has the advantages of stable performance, strong environmental adaptability, and strong insecticide and high temperature tolerance.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a Nannochloropsis mutant strain ENN11-4 and its application. Background technique [0002] In recent years, global oil resources are becoming increasingly depleted, energy shortages are becoming more and more serious, and environmental pollution and ecological damage are becoming more and more serious. The development of green and clean biofuels to replace traditional fossil fuels has become an international research hotspot. Among the many raw materials for biofuels, microalgae have the characteristics of high photosynthetic efficiency, short growth cycle, high oil content, strong environmental adaptability, and easy genetic improvement, and are considered to be the most potential biomass resources to replace petroleum. Microalgae biomass can not only produce biodiesel, high-quality aviation gasoline and other biofuels through biosmelting, but also develop products such as platform...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12P7/64C02F3/32A23L33/00A23K10/18A01K97/04A61K36/05C12R1/89
CPCA01K97/045C12N1/12C12P7/6409C12P7/6427C12N1/125C12R2001/89
Inventor 李青张蕊吴洪罗少敬崔春莉
Owner ENN SCI & TECH DEV
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