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Method for extracting chlorogenic acid from honeysuckle flower

A technology of chlorogenic acid and honeysuckle, applied in chemical instruments and methods, separation/purification of carboxylic acid esters, pharmaceutical formulations, etc., can solve problems such as unfavorable to industrial production, easy to volatilize into the environment, harm to human body and environment, etc. Achieve the effect of short extraction time, simple operation and good repeatability

Inactive Publication Date: 2015-01-21
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cost of supercritical extraction is too high to be conducive to industrial production
Although the ultrasonic method is relatively simple, ultrasonic extraction is generally carried out in an open system. Extraction solvents such as organic solvents are easy to volatilize into the environment during the extraction process, causing harm to the human body and the environment.

Method used

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  • Method for extracting chlorogenic acid from honeysuckle flower
  • Method for extracting chlorogenic acid from honeysuckle flower
  • Method for extracting chlorogenic acid from honeysuckle flower

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The present embodiment extracts the method for chlorogenic acid from honeysuckle, comprises the following steps:

[0025] (1) drying the raw material honeysuckle at a temperature of 50° C. for 2 hours, and pulverizing it to 100 mesh to obtain honeysuckle powder;

[0026] (2) Place the honeysuckle powder in the step (1) in a polytetrafluoroethylene reactor, add a methanol solution with a concentration of 25%, and the solid-liquid ratio is 1:20g / mL;

[0027] (3) the pH value of the mixed solution in step (2) is adjusted to 4 with dilute hydrochloric acid;

[0028] (4) The reaction kettle is placed in an oven, the oven temperature is set to be 110° C., and the extraction time is 30 minutes;

[0029] (5) Take the extract in step (4) and centrifuge at a high speed of 5000r / min for 3 minutes, and take the supernatant.

Embodiment 2

[0031] The present embodiment extracts the method for chlorogenic acid from honeysuckle, comprises the following steps:

[0032] (1) drying the raw material honeysuckle at a temperature of 50° C. for 2 hours, and pulverizing it to 100 mesh to obtain honeysuckle powder;

[0033] (2) Place the honeysuckle powder in the step (1) in a polytetrafluoroethylene reactor, add a methanol solution with a concentration of 50%, and the solid-liquid ratio is 1:20g / mL;

[0034] (3) the pH value of the mixed solution in step (2) is adjusted to 6 with dilute hydrochloric acid;

[0035] (4) The reaction kettle is placed in an oven, the oven temperature is set to be 110° C., and the extraction time is 30 minutes;

[0036] (5) Take the extract in step (4) and centrifuge at a high speed of 5000r / min for 3 minutes, and take the supernatant.

Embodiment 3

[0038] The present embodiment extracts the method for chlorogenic acid from honeysuckle, comprises the following steps:

[0039] (1) drying the raw material honeysuckle at a temperature of 50° C. for 2 hours, and pulverizing it to 80 mesh to obtain honeysuckle powder;

[0040] (2) Place the honeysuckle powder in the step (1) in a polytetrafluoroethylene reactor, add a methanol solution with a concentration of 25%, and the ratio of solid to liquid is 1:10g / mL;

[0041] (3) the pH value of the mixed solution in step (2) is adjusted to 6 with dilute hydrochloric acid;

[0042] (4) The reaction kettle is placed in an oven, the oven temperature is set to be 130° C., and the extraction time is 30 minutes;

[0043] (5) Take the extract in step (4) and centrifuge at a high speed of 5000r / min for 3 minutes, and take the supernatant.

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Abstract

The invention discloses a method for extracting chlorogenic acid from honeysuckle flower, and belongs to the technical field of extraction of effective components from medicinal plants. The method adopts a methanol solution to extract chlorogenic acid from honeysuckle flower at a temperature of 70-130DEG C under closed conditions to solve the disadvantages of a water extraction method, an ethanol reflux method, a supercritical extraction method and an ultrasonic extraction method. The method disclosed in the invention includes the following steps: 1, adding crushed honeysuckle flower powder of 80-100 meshes into a methanol solution with the concentration of 25-75%, and adjusting the pH value of the obtained mixed solution to 4-7; and 2, extracting under closed conditions for 10-60min, centrifuging, taking the obtained supernatant, and detecting the extraction rate of chlorogenic acid to be 3.32-4.19%. The extraction method has the advantages of simple operation, short extraction time, good repeatability, high stability, and avoiding of environment pollution caused by organic solvent volatilization due to the implementation of the whole extraction process in a closed system.

Description

technical field [0001] The invention relates to a method for extracting chlorogenic acid from honeysuckle, and belongs to the technical field of extracting effective components of medicinal plants. Background technique [0002] Honeysuckle is the dried flower bud of Lonicera Lonicerae Lonicera, which is an important and commonly used Chinese medicinal material in clinical practice in my country. There are more than 200 kinds of Lonicera plants in the Lonicera family in the world, and there are 98 kinds in my country, which are distributed in various provinces and regions of the country, and 47 kinds of them can be used for medicinal purposes. Honeysuckle is rich in a variety of nutrients beneficial to human health, such as volatile oils, flavonoids, chlorogenic acids, triterpenoids and trace elements. In recent years, chemical and pharmacological studies have confirmed that chlorogenic acid is one of the most pharmacologically active components in honeysuckle, and it is the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/355A61K133/00
CPCC07C67/48C07C67/56C07C2601/14C07C69/732
Inventor 李杰逄显娟王建刚王学廷和素娜吴银萍杨晖王利辉
Owner HENAN UNIV OF SCI & TECH
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