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Dendritic cell, preparation method thereof and application

A technology of dendritic cells and cells, applied in the field of biomedicine, can solve problems such as liver cancer treatment methods that need to be improved

Active Publication Date: 2015-01-21
WUHAN HAMILTON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the existing treatment methods for liver cancer still need to be improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Autologous blood DC-CIK immune cell therapy for patients with intrahepatic cholangiocarcinoma after surgery, the details are as follows:

[0057] 1. Clinical data

[0058] Case 1, female, 69 years old, had intrahepatic cholangiocarcinoma, confirmed by pathological examination after surgery.

[0059] 2. Main materials

[0060] (a) Consumables: lymphocyte separation medium (GE healthcare), GT-T551 medium (purchased from Baoriyi Biotechnology Co., Ltd.), rhGM-CSF, rhIL-4, rhIL-2, IL-β, IL-6 , IFN-γ, TNFα, alpha-fetoprotein, hepatitis B vaccine, CD3 monoclonal antibody (Beijing Yuance Pharmaceutical Co., Ltd.), 50ml centrifuge tube, 10ml centrifuge tube, 5ml pipette, 25ml pipette, Pasteur tubule , cell culture flask, cell culture bag (Thermo), gentamicin (purchased from Yichang Renfu Pharmaceutical Co., Ltd.).

[0061] (b) Instruments: flow cytometer (Beckman), biological safety cabinet (Likang), cell incubator (Likang), high-speed centrifuge (Kubota), inverted microscop...

Embodiment 2

[0090] Autologous blood DC-CIK immune cell therapy for patients with primary liver cancer stage II, the specific content is as follows:

[0091] 1. Clinical data

[0092] Case 2, male, 66 years old, stage II primary liver cancer, confirmed by pathological examination after surgery.

[0093] 2. Main materials

[0094] (a) Consumables: lymphocyte separation medium (GE healthcare), GT-T551 medium (sourced from Baoriyi Biotechnology Co., Ltd.), rhGM-CSF, rhIL-4, rhIL-2, IL-β, IL-6 , IFN-γ, TNFα, hepatitis B vaccine, CD3 monoclonal antibody (Beijing Yuance Pharmaceutical Co., Ltd.), 50ml centrifuge tube, 10ml centrifuge tube, 5ml pipette, 25ml pipette, Pasteur tubule, cell culture bottle , cell culture bag (Thermo fisher), gentamicin (from Yichang Renfu Pharmaceutical Co., Ltd.).

[0095] (b) Flow cytometer (bekman), biological safety cabinet (Likang), cell incubator (Likang), high-speed centrifuge (Kubota), inverted microscope (Jiangnan), ultra-low temperature refrigerator (Hai...

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Abstract

The invention provides a dendritic cell, a preparation method thereof and application. Concretely, the invention provides application of combination of the dendritic cell and cytokine-induced killer cell to prepare a medicine for treating or inhibiting liver cancer, wherein the dendritic cell is capable of specifically recognizing liver cancer nidus. According to the embodiment in the invention, the combination of the dendritic cell and the cytokine-induced killer cell is capable of effectively treating or inhibiting liver cancer.

Description

technical field [0001] The present invention relates to the field of biomedicine, specifically, the present invention relates to dendritic cells and their preparation method and use, more specifically, the present invention relates to the combination of dendritic cells and cytokine-induced killer cells in the preparation of medicines Use, method for preparing dendritic cells, medicine and dendritic cells. Background technique [0002] Liver cancer is the second cancer killer in China, with more than 300,000 deaths every year, accounting for 55% of the total number of liver cancer deaths worldwide. At present, the main means of treatment of liver cancer are surgical resection, liver transplantation, local ablation therapy, hepatic artery interventional therapy, radiation therapy, chemotherapy and other treatments. [0003] It is also found in any part of the world that chronic liver disease from any cause may play an important role in the occurrence and development of liver ...

Claims

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Application Information

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IPC IPC(8): A61K35/17C12N5/0784A61P35/00A61P1/16A61P31/20A61K35/16
CPCA61K35/16A61K35/17C12N5/0639A61K2300/00
Inventor 姚惟琦武栋成
Owner WUHAN HAMILTON BIOTECH
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