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Process for high-efficiency anaerobic production of acetic acid with homoacetogenic bacteria-rich seed sludge

A homoacetogenic bacteria and acetogenic technology, applied in the field of anaerobic high-efficiency acetogenic production, can solve the problems of many substances and complex processes, and achieve the effect of simple process and low energy consumption

Inactive Publication Date: 2015-01-07
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using its microbial degradation of organic matter to achieve waste reduction treatment and high-yield value-added products, the process is complex and complex, especially the degradation of organic matter by acid-producing bacteria has not been used in large-scale waste treatment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] In the UASB reactor whose effective volume is 5L, add the seed mud of 114.94g, make the seed mud concentration in the reactor be 7g / L (kind of mud VS is 30.45%), add COD and be the molasses waste water of 7000mg / L as substrate, The initial pH value of the reactor solution was adjusted to 8 by adding an appropriate amount of 2mol / L NaOH. After 35 days of fermentation, the acetic acid and total acid contents were 3.63734g / L and 7.115995g / L, and the proportion of acetic acid was 51.11%. COD removal rate is 89.2%.

Embodiment 2

[0024] In the UASB reactor whose effective volume is 5L, add the seed mud of 114.94g to make the seed mud concentration in the reactor be 7gVS / L (the seed mud VS is 30.45%), add the molasses wastewater that COD is 10000mg / L and add the reactor as the bottom The initial pH value of the reactor solution was adjusted to 8 by adding an appropriate amount of 2mol / L NaOH. After 35 days of fermentation, the contents of acetic acid and total acid were 5.615425g / L and 7.115995g / L, of which acetic acid accounted for 63.89%, and the COD removal rate was 92.4%.

Embodiment 3

[0026] In the UASB reactor whose effective volume is 5L, add the seed mud of 82.10g to make the seed mud concentration in the reactor be 5gVS / L (the seed mud VS is 30.45%), and adding COD is molasses wastewater of 10000mg / L to add to the reactor as the bottom The initial pH value of the reactor solution was adjusted to 8 by adding an appropriate amount of 2mol / L NaOH. After 35 days of fermentation, the contents of acetic acid and total acid were 6.48868g / L and 10.942375g / L, of which acetic acid accounted for 59.30%, and the COD removal rate was 94.0%.

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Abstract

The invention relates to a technology for high-efficiency anaerobic production of acetic acid by waste water, and particularly relates to a process for high-efficiency production of acetic acid with homoacetogenic bacteria-rich seed sludge through adjusting the reaction conditions and substrate concentration under anaerobic conditions. According to the process, anaerobic fermentation of waste water is carried out in a UASB reactor by using homoacetogenic bacteria-rich seed sludge, during which a trace element culture solution is added in the UASB reactor to keep the activity of microbes. The activities of methanogens are restrained and the activities of hydrogen-producing acetogens and homoacetogenic bacteria are improved through the adjustment of the initial pH value, the change of the waste water substrate concentration and the variation of the concentration of the seed sludge during the anaerobic fermentation of the sludge, thereby producing acetic acid with high efficiency instead of the low-added-value product methane and finally achieving the purpose of maximally utilizing waste resources.

Description

technical field [0001] The invention relates to waste water resource utilization technology, in particular to a process for anaerobic and high-efficiency acetogenic production using sludge enriched with homoacetogenic bacteria under optimum physical and chemical conditions and substrate concentration. Background technique [0002] Homoacetogens are a type of anaerobic microorganisms that can reduce two molecules of carbon dioxide to acetic acid through the anaerobic acetyl-CoA pathway. Yield up to 100%. Homoacetogenic bacteria are a kind of microorganisms with wide distribution and great differences in morphology. They can live in both autotrophic and heterotrophic forms. They account for 3%-11% of the total microbial mass in anaerobic sludge; they form approximately 1013kg acetic acid, accounting for 10% of the entire anaerobic acid production system; therefore homoacetogenic bacteria occupy a very important position in the global carbon cycle process, and have great poten...

Claims

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Application Information

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IPC IPC(8): C12P7/54
CPCC12P7/54
Inventor 王晋李习伟林超杨彦李定龙
Owner CHANGZHOU UNIV
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