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Novel application of arabidopsis TTG1 genes to plant salinity-resisting and drought-resisting aspects

A kind of Arabidopsis thaliana, newly applied technology, applied in the field of genetic engineering application

Inactive Publication Date: 2015-01-07
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on other uses of this gene, especially research on plant drought resistance

Method used

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  • Novel application of arabidopsis TTG1 genes to plant salinity-resisting and drought-resisting aspects
  • Novel application of arabidopsis TTG1 genes to plant salinity-resisting and drought-resisting aspects
  • Novel application of arabidopsis TTG1 genes to plant salinity-resisting and drought-resisting aspects

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Experimental program
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Effect test

Embodiment

[0018] (1) Verification TTG1 Gene (At5g24520 gene) gene is related to plant salt and drought resistance

[0019] to prove TTG1 The gene (At5g24520 gene) is related to the salt and drought resistance of plants. The inventors used Arabidopsis wild type (WT), ttg1-1 and ttg1-2 The mutants were verified by comparative experiments. said ttg1-1 and ttg1-2 Mutants were obtained from the Arabidopsis Resource Center TTG1 gene (At5g24520 gene) T-DNA insertion mutant (CS89, CS67772), the mutant's TTG1 The gene is inactive and cannot correctly encode the associated protein.

[0020] The specific experimental verification process is briefly introduced as follows:

[0021] Arabidopsis wild type (WT), ttg1-1 and ttg1-2 The mutant seeds were sown on MS medium containing 100 mM NaCl, 200 mM mannitol, and 0.1 μM ABA, and the MS medium control without other substances was set, and the mass content of MS medium agar was 0.6%.

[0022] Arabidopsis thaliana was cultured and grown u...

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Abstract

The invention belongs to the technical field of gene engineering application, and particularly relates to application of arabidopsis TTG1 genes to cultivation of new salinity-resisting and drought-resisting plant varieties. A code of arabidopsis genomes is At5g24520. By means of the genes, arabidopsis can tolerate the salinity stress or drought stress growing environment; plants with the genes transformed can tolerate the salinity stress or drought stress growing environment. According to a series of experimental studies, it is found that regulation plants with the gene specificity show salinity-resisting and drought-resisting characteristics on osmotic stress reactions caused by salinity and drought; the huge potential application function of the genes on the plant salinity-resisting and drought-resisting aspects is proved; new transgenic plant varieties related to the salinity stress resistance and the drought stress resistance can be obtained; the salinity-resisting and drought-resisting functions of the plants are achieved accordingly. Theoretical and production practice foundations are laid for cultivating the new salinity-resisting and drought-resisting transgenic crop varieties.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering application, in particular to Arabidopsis thaliana TTG1 The application of the gene (Arabidopsis thaliana genome coded as At5g24520) in breeding new varieties of salt- and drought-tolerant plants. Background technique [0002] At present, the shortage of global water resources is increasing day by day, and the extensive use of chemical fertilizers by humans has caused soil salinization to become increasingly serious, restricting the sustainable production and development of agriculture. With the deepening of molecular biology and genetic engineering research, many scientific and technological workers in the world are also trying to find genes related to plant salt resistance. They plan to improve the salt resistance of crops through genetic engineering technology, but there are very few achievements available at present. . [0003] Arabidopsis ( Arabidopsis thaliana ) belongs to th...

Claims

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Application Information

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IPC IPC(8): C12N15/29A01H5/00
CPCY02A40/132
Inventor 张骁赵翔王琳丹王进赵青平臧毅浩
Owner HENAN UNIVERSITY
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